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EC number: 942-293-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 February - 24 March 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed under GLP and according to guideline with some deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- No measurements of the concentrations. No data on Daphnia culture.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
- EC Number:
- 263-180-1
- EC Name:
- Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
- Cas Number:
- 61791-47-7
- Molecular formula:
- not applicable
- IUPAC Name:
- N,N-Bis(2-hydroxyethyl)-C12-18(even numbered) alkyl-1-amine oxides
- Details on test material:
- The test material was identified by name and specifications:
- chemical name: Cocobis(2-hydroxyethyl)amine oxide
- cas nr : 61791-47-7
- batch no: 900110 (CoA attached to the report):
Amine oxide: 30.5%
Free amine: 0.3%
Free peroxide: 0.13%
water: balance
CRL code T90-2-1.5
It was supplied by the study sponsor on 8 Feb 1990. Stored in the dark at room temperature.
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Liquid.
Stable at test concentrations (data on biodegradability available).
Water soluble at test concentrations.
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared from stock solutions of the test substance in deionized water. The stock solution was clear and homogeneous
(some foam was produced but disappeared after a short time). The test concentrations were prepared by addition of the respective amounts of the
stock solution to the DutchStandardWater. For the preparation of the test concentration a stock solution containing 0.3 g/L was used.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna (waterflea) originated from the continuous culture maintained at the research facilities. The animals used in the test were less than
24 hours old at the beginning of the test and were obtained from parent animals having an age of about 3 weeks.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 13 dH
- Test temperature:
- 19.3 - 19.7 C
- pH:
- 8.0 - 8.1
- Dissolved oxygen:
- 8.8 - 9.1 mg/L
- Nominal and measured concentrations:
- Nominal concentrations:
0.46 - 1.0 - 2.1 - 4.6 - 10 mg/L - Details on test conditions:
- 400 mL glass beakers containing 250 mL of test medium were partly covered with a glass plate. Each beaker contained 5 animals. In the control 30
animals were tested, while 20 animals were tested per test concentration.
The diluting water used for the preparation of the test concentrations was a synthetic water: Dutch Standard Water (DSW): ph = 8.2 and a hardness of 13 dH. The test was carried out in a temperature controlled room. The light regime was 16 hours of ambient light per day, supplied by fluorescent
tubes. pH and oxygen concentrations were measured daily.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 0.9-1.3 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 2.9 - 4.3 mg/L
- Details on results:
- The NOEC after 48 hours amounted to 0.46 mg/L, whereas 10 mg/L produced 100% immobilization within 48 hours. The EC50 (48h) was calculated
to be 3.5 mg/L with 95% confidence limits of 2.9 - 4.3 mg/L. - Reported statistics and error estimates:
- The EC50 was determined with an LC50 programme of Griffioen (RIZA, NL) based on a model of Kooyman (1981).
Any other information on results incl. tables
Number of mobile animals
Conc substance |
Test vessel |
|||
in mg/l |
number |
0 hours |
24 hours |
48 hours |
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
3 |
5 |
5 |
5 |
|
control |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
6 |
5 |
5 |
5 |
|
total |
30 |
30 |
30 |
|
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
0.46 |
3 |
5 |
5 |
5 |
4 |
5 |
5 |
5 |
|
total |
20 |
20 |
20 |
|
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
4 |
|
1.0 |
3 |
5 |
5 |
5 |
4 |
5 |
5 |
5 |
|
total |
20 |
20 |
19 |
|
1 |
5 |
5 |
5 |
|
2 |
5 |
5 |
5 |
|
2.1 |
3 |
5 |
5 |
4 |
4 |
5 |
5 |
5 |
|
total |
20 |
20 |
19 |
|
1 |
5 |
5 |
1 |
|
2 |
5 |
5 |
0 |
|
4.6 |
3 |
5 |
4 |
1 |
4 |
5 |
5 |
3 |
|
total |
20 |
19 |
5 |
|
1 |
5 |
4 |
0 |
|
2 |
5 |
3 |
0 |
|
10 |
3 |
5 |
5 |
0 |
4 |
5 |
4 |
0 |
|
total |
20 |
16 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50(48h) was calculated to be 3.5 mg/L with 95% confidence limits of 2.9 and 4.3 mg/L. The NOEC (48h) was 0.46 mg/L. If it is assumed that the product has a 100% pure active ingredient and that the toxicity is related to the active ingredient only, the EC50 and NOEC (48h) amount to 1.1 and 0.14 mg/L amine oxide respectively.
- Executive summary:
A static study was performed according to OECD203 and GLP to investigate the acute toxicity of the test item to the aquatic invertebrate Daphnia magna. Based on nominal concentrations only, the EC50 was calculated to be 3.5 mg/L with 95% confidence limits of 2.9 and 4.3 mg/L. The NOEC after 48h was 0.46 mg/L.
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