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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Short term toxicity of test chemical were studied on the mortality of fish.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 24 hrs continuous stirring for achieving test concentrations of 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.86 cm
- Weight at study initiation (mean and range, SD): average 0.193 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
149.5 mg of CaCO3
Test temperature:
24.1 °C
pH:
7.1
Dissolved oxygen:
7.7 mg/l
Nominal and measured concentrations:
Nominal concentrations : 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Table 1. Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.3125

4

4

-

-

-

-

-

-

-

-

-

-

-

-

 0.625  4  4  -  -  -  -  -  -  -  -  -  -  -  -
 1.25  4  4  -  -  -  -  -  -  -  -  -  -  -  -
 2.5  4  4  -  -  -  -  -  -  -  -  -  -  -  -
 5  4  4  -  -  -  -  -  -  1  1  2  2  2  2

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.3125

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.625

 4

 4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

1.25

 4

 4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 2.5

 4

 4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

 NS4

5.0

 4  4  NS4  NS4  NS4  NS4  NS4  NS4  NS3  NS3  NS2  NS2  NS2  NS2

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:
yes
Conclusions:
The study concludes that the test chemical is toxic to the aquatic environment at a median lethal concentration (LC50) of 5.0 mg/L.
Executive summary:

The study outlines the effect of test chemical on the aquatic test modeldanio rerio. This test was performed by following the OECD guidelines 203- Fish Acute Toxicity Test. Five different test concentrations was taken ranging from 0.3125 to 5 mg/L by following the geometric series of factor 2. The test was performed for 96 hrs, and mortality was recorded for every interval of 24 hours up to 96 hours.

Based on the mortality observed in exposed tenure, the observed median lethal concentration of the test chemical is (LC50) 5.0mg/L. The test chemical is toxic to the aquatic environment and it can be classified as aquatic chronic category 2. 

Description of key information

The study outlines the effect of test chemical on the aquatic test modeldanio rerio. This test was performed by following the OECD guidelines 203- Fish Acute Toxicity Test. Five different test concentrations was taken ranging from 0.3125 to 5 mg/L by following the geometric series of factor 2.

The test was performed for 96 hrs, and mortality was recorded for every interval of 24 hours up to 96 hours.

Based on the mortality observed in exposed tenure, the observed median lethal concentration of the test chemical is (LC50) 5.0mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
5 mg/L

Additional information

The study outlines the effect of test chemical on the aquatic test modeldanio rerio. This test was performed by following the OECD guidelines 203- Fish Acute Toxicity Test. Five different test concentrations was taken ranging from 0.3125 to 5 mg/L by following the geometric series of factor 2. The test was performed for 96 hrs, and mortality was recorded for every interval of 24 hours up to 96 hours.

Based on the mortality observed in exposed tenure, the observed median lethal concentration of the test chemical is (LC50) 5.0mg/L. The test chemical is toxic to the aquatic environment and it can be classified as aquatic chronic category 2.