Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
6.06.2000-10.06.2000
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline

Test solutions

Details on test solutions:
10, 20, 50, 100, 200, 500mg/l

Test organisms

Test organisms (species):
other: Scophthalmus maximus
Details on test organisms:
TEST ORGANISM
- Common name: turbut (Danish: Pighvar)
- Strain: Scophthalmus maximus
- Source: Kommercial producer in Thisted
- Age at study initiation (mean and range, SD):
- Length at study initiation: 57-65mm (A little above the PARCOM recommended size: 40-60mm)
- Weight at study initiation: 6.9g

ACCLIMATION
- Acclimation period: 8 d
- Acclimation conditions: same as test
- Type of food: trout forage
- Health during acclimation: No mortality observed

Study design

Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
24, 48, 72 & 96 h after exposure.

Test conditions

Test temperature:
20±2ºC
pH:
8±0.3
Dissolved oxygen:
> 70%
Details on test conditions:
TEST SYSTEM
- Test vessel: 10L polyethylen aquarium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates):1 without test substance 1 with reference test substance K2Cr2O

OTHER TEST CONDITIONS
- Photoperiod: 12:12 h light/dark

TEST CONCENTRATIONS
- Test concentrations: 10, 20, 50, 100, 200, 500 mg/l
Reference substance (positive control):
yes
Remarks:
Sodiumdichromate, K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LOEC
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC10
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality observed
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- LC50(48h): 311 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Mortality in controls < 10 %, oxygen > 60%
Conclusions:
The substance has a LC50 (96h) > 500 mg/l for Scophthalmus maximus. No death was observed.