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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
SALMONELLA MUTAGENICITY TESTS: IV. RESULTS FROM THE TESTING OF 300 CHEMICALS;
Author:
ZEIGER,E, ANDERSON,B, HAWORTH,S, LAWLOR,T AND MORTELMANS,K;
Year:
1988
Bibliographic source:
ENVIRON. MOL. MUTAGEN. 11(SUPPL.12):1-158, 1988
Reference Type:
other: Authoritative data base
Title:
Study ID : 241943
Author:
NTP Database
Year:
2012
Bibliographic source:
NTP (National Toxicological Program)by Agency for Toxic Substances and Disease Registry; Division of Toxicology/Toxicology Information Branch, 1600 Clifton Road NE, E-29, Atlanta, Georgia 30333
Reference Type:
other: Authoritative data base
Title:
GCID : 512737
Author:
ZEIGER,E, ANDERSON,B, HAWORTH,S, LAWLOR,T AND MORTELMANS,K
Year:
2011
Bibliographic source:
ACToR (Aggregated Computational Toxicology Resource):ZEIGER,E, ANDERSON,B, HAWORTH,S, LAWLOR,T AND MORTELMANS,K; SALMONELLA MUTAGENICITY TESTS: IV. RESULTS FROM THE TESTING OF 300 CHEMICALS; ENVIRON. MOL. MUTAGEN. 11(SUPPL.12):1-158, 1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
PREINCUBATION : In the standard protocol (preincubation) for conducting the Ames assay, a test tube containing a suspension of one strain of Salmonella typhimurium (or E. coli) plus S9 mix or plain buffer without S9, is incubated for 20 minutes at 37º C with the test chemical. Control cultures, with all the same ingredients except the test chemical, are also incubated. In addition, positive control cultures are prepared; these contain the particular bacterial tester strain under investigation, the various culture ingredients, and a known potent mutagen*. After 20 minutes, agar is added to the cultures and the contents of the tubes are thoroughly mixed and poured onto the surface of Petri dishes containing standard bacterial culture medium. The plates are incubated, and bacterial colonies that do not require an excess of supplemental histidine appear and grow. These colonies are comprised of bacteria that have undergone reverse mutation to restore function of the histidine-manufacturing gene. The number of colonies is usually counted after 2 days.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
3-diethylaminophenol
EC Number:
202-090-9
EC Name:
3-diethylaminophenol
Cas Number:
91-68-9
Molecular formula:
C10H15NO
IUPAC Name:
3-(diethylamino)phenol
Test material form:
solid: crystalline
Details on test material:
- Name of test material (as cited in study report): 3-diethylaminophenol
- Substance type: Organic
- Physical state: Solid

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 100
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 1535
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 97
Additional strain / cell type characteristics:
not specified
Species / strain / cell type:
S. typhimurium TA 98
Additional strain / cell type characteristics:
not specified
Metabolic activation:
without
Metabolic activation system:
none
Test concentrations with justification for top dose:
10-666 UG/PLATE
Vehicle / solvent:
DMSO
Details on test system and experimental conditions:
Test System : AMES SALMONELLA TYPHIMURIUM

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 100
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium TA 97
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium TA 98
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative without metabolic activation

In an Ames test , 3-diethylaminophenol, in dimethyl sulfoxide from doses 10-666 UG/PLATE was not mutagenic in Salmonella typhimurium strains TA100,TA1535,TA97 and TA98 without metabolic activation.
Executive summary:

In an Ames test , 3-diethylaminophenol, in dimethyl sulfoxide from doses 10-666 UG/PLATE was not mutagenic in Salmonella typhimurium strains TA100,TA1535,TA97 and TA98 without metabolic activation.