Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-852-7
CAS number: 624-54-4
A 96-hour preliminary range-finding exposure
was conducted at Springborn Smithers Laboratories (Europe) at nominal
UCAR n-Pentyl Propionate concentrations of 0.020, 0.20, 2.0, 20 and 200
mg test item/l and a control. Three 100 -ml glass serum bottles crimped
sealed with aluminum seals containing Teflon-lined rubber septa were
established for each concentration and the control. Following 96 hours
of exposure, cells exposed to the 0.020, 0.20, 2.0 and 20 mg test item/l
and the control were observed to be normal. In the highest treatment
level ciliates were observed. Cell densities in the control and 0.020,
0.20, 2.0, 20 and 200 mg test item/l treatment levels averaged 277, 273,
278, 279, 108 and 0.8 x 104 cells/ml, respectively.
Based on these results, nominal
concentrations of 1.9, 6.1, 19.5, 62.5 and 200 mg test item/l and a
control were selected for the definitive exposure.
The pH of the test and control solutions
ranged from 7.36 to 7.47 at test initiation. After 72 and 96 hours of
exposure, pH measured in the composited test solutions of the treatment
levels and the control ranged from 7.10 to 10.13 and from 6.80 to 11.04,
respectively. The low pH value measured in the highest test
concentration at test completion was most likely due to no cell growth
and/or the degradation of the test item over the 96 hour test duration.
Continuous temperature monitoring established a temperature range from
22 to 25°C during the test period. Light intensity of
the test area ranged from 3921 to 4543 lux. The shaking rate was
maintained throughout the exposure at 100 rpm.
After preparation of the test solution at
day 0, the percentage recoveries ranged from 66.5 to 74.7% of the
nominal concentrations. After 96 hours of exposure, recoveries of below
the limit of quantification (LOQ) of 1.72 mg test item/L were found for
all treatment levels. The 96-hour recovery of the 19.5 mg test item/L
solutions without algae (abiotic control) was also below the LOQ. Based
on this, it can be concluded that the reduction in concentration at hour
72 was not caused (only) by the presence of algae cell.
Based on these results, initial measured
concentrations of 1.4, 4.5, 13.0, 45.2 and 141 mg test item/L were used
for the evaluation of the biological data.
Analysis of the QC samples prepared in
dilution water resulted in measured concentrations ranging from 90.7 to
114% (N = 6) of nominal concentration at hour 0 and 96. The results of
the QC samples established that the appropriate quality control was
maintained during the analyses of the exposure solutions.
The purpose of this study was to determine
the effects of UCAR (n-Pentyl Propionate) on the growth of Pseudokirchneriella
subcapitata under static conditions in a closed-bottle system. The
results are reported as the 72- and 96-hour No-Observed-Effect
Concentration (NOEC) and Lowest-Observed-Effect Concentration (LOEC)
values for yield and growth rate. Additionally, the 72- and 96-hour
EC10, EC20, EC50 values for both endpoints, and the 24- and 48-hour EC50
values for yield were determined. The nominal test concentrations were -
Control, 1.9, 6.1, 19.5, 62.5 and 200 mg test item/l (initial measured
concentrations were - Control, 1.4, 4.5, 13.0, 45.2 and 141 mg test
Based on initial measured concentrations (mg
test item/L), the 72- and 96-hour (growth rate) ErC50 values for
n-pentyl propionate to Pseudokirchneriella subcapitata (syn. Selenastrum
capricornutum) under static conditions in a closed system were 41.1
and 41.7 (initial measured concentration) mg/l with 95 % confidence
limits ranging between 39.6 - 43.1 and 40.3 - 44.2 mg/l, respectively.
The 72 and 96 -hour NOEC values were both 4.5 mg/L.
In a GLP study conducted according to OECD TG 201, the 72-hour ErC50 (growth rate) of pentyl propionate to Pseudokirchneriella subcapitata was 41.1 mg/l with 95% fiducial limits ranging between 39.6 - 43.1 mg/l and the 72-hour NOEC was 4.5 mg/l
In a GLP study conducted according to OECD
TG 201, the 72 -hour ErC50 (growth rate) of pentyl propionate to Pseudokirchneriella
subcapitata was 41.1 mg/l with 95% confidence limits ranging between
39.6 -43.1 mg/l, while the 96 -hour ErC50 (growth rate) was 41.7 (95% CL
39.6 - 43.1). Both the 72 -hour and 96 -hour NOEC values were 4.5 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again