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EC number: 242-893-1 | CAS number: 19223-55-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 november 2012 - 13 march 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples were taken from each treatment just before the start of the first and of the last renewal periods (Days 0 and 3). At the end of these renewal periods (Days 1 and 4), additional samples were taken for determination of the stability of the test item during the renewal periods of 24 hours. All samples were taken from the approximate center of each aquarium without mixing the test medium, and were stored deep-frozen (at about -20 °C) immediately after sampling. The concentrations of the test item were determined in one of the duplicate test medium samples from the nominal test concentrations of 28.1 mg/L from all sampling times.
From the control samples, only one of the duplicate samples of the freshly prepared test medium was analyzed per sampling time. The control samples of the aged test medium were not analyzed. - Vehicle:
- no
- Details on test solutions:
- At the start of the test and before each test medium renewal, the test medium of nominal 28.1 mg/L was freshly prepared by completely dissolving 0.1407, 0.1407, 0.1404 and 0.1405 mg of the test item in five liter of test water under intense stirring for 15 minutes at room temperature, respectively. The test medium was freshly prepared just before introduction of the fish (= start of the exposure and prior to each test medium renewal).
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish.
- Strain: Danio rerio.
- Source: breeding culture at Harlan Laboratories.
- Length at study initiation (length definition, mean, range and SD): 2.81 ± 0.14 cm (Mean ± SD).
- Weight at study initiation (mean and range, SD): 0.15 ± 0.03 g (Mean ± SD).
- Feeding during test: no.
ACCLIMATION
- Acclimation period: one week prior to test.
- Acclimation conditions: same as test.
- Type and amount of food: commercial fish diet.
- Health during acclimation: during holding and acclimatization no mortality observed and all fish were healthy. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 125 mg/L as CaCO3
- Test temperature:
- 21°C
- pH:
- 7.1 to 7.5
- Dissolved oxygen:
- At least 8.1 mg/L (corresponding to at least 90% oxygen saturation)
- Nominal and measured concentrations:
- Nominal concentration: 28.1 mg test item/L (limit test) corresponding to 12.9 mg solid content/L and 10 mg active content/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: one glass vessel with 5 liters of test medium for each treatment.
- Type: open.
- Aeration: slight aeration during the test.
- Renewal rate of test solution (frequency/flow rate): test media was renewed daily during the test period of 96 hours.
- No. of organisms per vessel: 7.
- No. of vessels per concentration (replicates): 1.
- No. of vessels per control (replicates): 1.
- Biomass loading rate: 0.21 g fish wet weight per liter test medium.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water. It consisted of analytical grade salts dissolved in purified water.
- Alkalinity: 0.4 mmol/L.
- Ca/Mg ratio: 4:1.
- Na/K ratio: 10:1.
- Intervals of water quality measurement: the water temperature, pH values and oxygen concentrations were measured for each treatment at the start of the test and once daily during the test in the freshly prepared and old test media.
OTHER TEST CONDITIONS
- Adjustment of pH: no.
- Photoperiod: 16 hour light to 8-hour dark photoperiod, with a 30-minute transition period was used.
- Light intensity: 140 to 480 Lux.
EFFECT PARAMETERS MEASURED: the test fishes were observed for mortality and visible abnormalities after approximately 4, 24, 48, 72 and 96 hours test duration.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test / one concentration tested.
- Range finding study: no.
- Test concentrations: 28.1 mg/L (test item). - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 28.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 12.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: solid content
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 28.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 12.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: solid content
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Details on results:
- Behavioural abnormalities: no.
Mortality of control: no.
In the test medium sample taken at the start and end of the first and last test medium renewal periods, the measured test item concentration ranged between 94 and 118% of the nominal value (less than 20% of deviation from nominal concentration). The oxygen concentration was at least 8.1 mg/L (see table 3) corresponding to at least 90% oxygen saturation.
All the validity criteria were fulfilled:
- mortality of controls is less than 10% at the end of the test;
- test conditions (pH, temperature) were maintained during the test (see tables 1 and 3);
- the test item concentration was maintained during the test (less than 20% of deviation from nominal concentration);
- the oxygen saturation was maintained during the test and was above 60% of oxygen saturation. - Sublethal observations / clinical signs:
Table 1 pH in the Freshly Prepared and Old Treatment Media
Nominal
test item concentration(mg/L)
Exposure time
0 h
24 h
48 h
72 h
96 h
new
old
new
old
new
old
new
old
Control
7.3
7.2
7.2
7.5
7.4
7.4
7.3
7.2
28.1
7.3
7.2
7.1
7.5
7.4
7.4
7.3
7.2
Table 2 Oxygen Concentrations (mg/L) in the Freshly Prepared and Old Treatment Media
Nominal
test item concentration(mg/L)
Exposure time
0 h
24 h
48 h
72 h
96 h
new
old
new
old
new
old
new
old
Control
9.8
8.4
9.6
8.5
9.9
8.4
9.7
8.2
28.1
9.7
8.4
9.7
8.5
9.9
8.5
9.6
8.1
Table 3 Temperatures (°C) in the Freshly Prepared and Old Treatment Media
Nominal
test item concentration(mg/L)
Exposure time
0 h
24 h
48 h
72 h
96 h
new
old
new
old
new
old
new
old
Control
21
21
21
21
21
21
21
21
28.1
21
21
21
21
21
21
21
21
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item exhibited no adverse effects on zebra fish at the nominal concentration of 28.1 mg/L (corresponding to 12.9 mg solid content/L and 10 mg active content/L).
- Executive summary:
The short-term aquatic toxicity of the REACh substance (2-Hydroxy-3-sulphopropyl) dimethyl[3-[(1-oxododecyl) amino]propyl]ammonium hydroxide (EC 242-893-1) was investigated in a GLP-compliant study performed with the freshwater fish species Danio rerio (zebra fish) in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test) and EU Method C.1. The test item exhibited no adverse effects on zebra fish at the nominal concentration of 28.1 mg/L (limit test) and the 96h-LC50 value is reported to be greater than 28.1 mg/L (corresponding to 12.9 mg solid content/L and 10 mg active content/L).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 june 2009 - 13 july 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Despite the lack of analytical monitoring data, it can be reasonably assumed that the test concentrations remained stable over the test duration from the analytical measurements reported in the long-term toxicity test to Daphnia magna performed on a structural analogue substance (Höger, 2013).
- Qualifier:
- according to guideline
- Guideline:
- other: OSPAR / PARCOM Protocols on methods for the testing of chemicals used in the offshore Industry 2006
- Deviations:
- yes
- Remarks:
- fish length and loading rate above recommended maximum values
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Concentrations: 0 (control) and 10 mg test item/L.
- Method: samples were taken after 24, 48, 72 and 96 hours in order to observe and record mortalities. Water quality determinations for each test and control solution were carried out immediately prior to initiating the test, after 24 hours, before and after the 48 hour change of test solutions, after 72 hours and at the end of the 96 hour exposure period.
- Reference vessels: none.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution of 1000 mg/L (nominal) was prepared with dilution water, shaken vigorously and allowed to stand for four hours. For each nominal concentration the required amount of homogenized stock solution was added to 10 litres of dilution water. Dilution water is a dechlorinated water with Tropic Marin artificial sea salt formulated by Tropical Marine Centre Limited added to a final salinity of 33 to 35 g/L sodium chloride. Test solutions were replaced after 48 hours up to the maximum exposure period of 96 hours.
- Controls: culture medium without test item.
- Evidence of undissolved material (e.g; precipate, surface film, etc): no. - Test organisms (species):
- other: Scophthalmus maximus (turbot)
- Details on test organisms:
- TEST ORGANISM
- Common name: turbot.
- Strain: Scophthalmus maximus.
- Source: France Turbot, Noirmoutiers, France.
- Loading rate: 2.08 g fish/litre.
- Feeding during test: no.
- Length at study initiation: mean length of 58.7 mm.
- Weight at study initiation: mean weight of 2.97 g.
ACCLIMATION
- Acclimation period: ca. 2 month.
- Acclimation conditions: Scophthalmus maximus were maintained in dechlorinated mains water with Tropic Marin artificial sea salt formulated by Tropical Marine Centre Limited at 14.5 to 15.5°C, above 98% dissolved oxygen and under a photoperiod of 16 hours light and 8 hours dark.
- Type and amount of food: fed with Le Gouessant Turbot Label Rouge at a rate of approximately 2% body weight per day until 24 hours before test initiation.
- Feeding frequency: daily.
- Health during acclimation (any mortality observed): 0.4% mortality during acclimatation period. - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- no data
- Test temperature:
- 15.0 - 15.5°C
- pH:
- 8.2 - 8.3
- Dissolved oxygen:
- 83 - 101%
- Salinity:
- 32 ‰
- Nominal and measured concentrations:
- Nominal concentration: 10 mg test item/L (corresponding to 4.8 mg solid content/L).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 12 litre volume aquaria
- Type: open
- Aeration: no data
- Renewal rate of test solution (frequency/flow rate): test solutions replaced after 48 hours up to the maximum exposure period of 96 hours.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.08 g fish per litre
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water is a dechlorinated water with Tropic Marin artificial sea salt formulated by Tropical Marine Centre Limited added to a final salinity of 33 to 35 g/L sodium chloride.
- Culture medium different from test medium: No
- Intervals of water quality measurement: each 24 hours up to the end of the test after 96 hour exposure period.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours dark
EFFECT PARAMETERS MEASURED: The fish were observed for mortality and abnormal behaviour after 24, 48, 72 and 96 hours exposure periods.
TEST CONCENTRATIONS
Limit test at 10 mg/L (nominal) - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 4.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: solid content
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Details on results:
- Behavioural abnormalities: no.
Mortality of control: no.
The following validity criteria were fulfilled:
- mortality of controls is less than 10% at the end of the test;
- test conditions (pH, temperature, salinity) were maintained during the test;
- the oxygen saturation was maintained during the test and was above 60% of oxygen saturation.
There is a lack of information of data relating to the analytical monitoring showing that the concentrations of the test item were maintained during the test. However, according to the long-term toxicity test to Daphnia performed on a very similar substance, the substance is stable during 48 hours. Thus, we can consider that in a short-term study, the substance is also stable.
The largest fish tested was 65 mm and the loading rate was 2.08 g fish per litre while the guideline recommends a maximum fish length of 60 mm and a loading rate of 1 g fish per litre of test solution. Nevertheless, water qualities remains within recommended limits and no mortalities or abnormal behavior of the Turbot was recorded. Thus, this deviation is considered not to have adversely affected the data.
For details see tables 1 to 4 in ‘Any other information on results incl. tables’. - Sublethal observations / clinical signs:
Table 1: Summary of pH values
Exposure period (hours)
Concentration (mg/L)
Control
10.0
0
8.2
8.2
24
8.2
8.2
48
8.2
8.2
8.3
8.2
72
8.3
8.3
96
8.2
8.3
Table 2: Summary of dissolved oxygen values (% ASV)
Exposure period (hours)
Concentration (mg/L)
Control
10.0
0
101
100
24
100
99
48
99
98
101
100
72
99
99
96
83
92
Table 3: Summary of temperature values (°C)
Exposure period (hours)
Concentration (mg/L)
Control
10.0
0
15.0
15.0
24
15.5
15.5
48
15.0
15.0
15.0
15.0
72
15.5
15.5
96
15.5
15.5
Table 4: Summary of salinity (‰)
Exposure period (hours)
Concentration (mg/L)
Control
10.0
0
32
32
24
32
32
48
32
32
32
32
72
32
32
96
32
32
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item exhibited no adverse effects on turbot at the nominal concentration of 10 mg/L (corresponding to 4.8 mg solid content/L).
- Executive summary:
The short-term aquatic toxicity of the REACh substance (2-Hydroxy-3-sulphopropyl) dimethyl[3-[(1-oxododecyl) amino]propyl]ammonium hydroxide (EC 242-893-1) was investigated in a GLP-compliant study performed with the marine fish species Scophthalmus maximus (turbot) in accordance with OSPARCOM guidelines. The test item exhibited no adverse effects on turbot at the nominal concentration of 10 mg/L (limit test) and the 96h-LC50 value is reported to be greater than 10 mg/L (corresponding to 4.8 mg solid content/L).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 january 2009 - 24 march 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Despite the lack of analytical monitoring data, it can be reasonably assumed that the test concentrations remained stable over the test duration from the analytical measurements reported in the long-term toxicity test to Daphnia magna performed on a structural analogue substance (Höger, 2013).
- Qualifier:
- according to guideline
- Guideline:
- other: OSPARCOM (1995) (amended March 2005) - Protocol for a fish acute toxicity test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test solution was prepared without any organic solvent.
- Control: one replicate.
- Reference vessels: no reference vessels.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test item is soluble in water and was added to the test system directly via seawater.
- Stock solution: no. At the beginning of the test, 0.03884 g of test item was diluted to 23000 ml of treated seawater. The same tested solution was generated at 48h.
- Controls: no data.
- Evidence of undissolved material (e.g; precipate, surface film, etc): no. - Test organisms (species):
- other: Scophthalmus maximus (turbot)
- Details on test organisms:
- - Common name: turbot.
- Strain: Scophthalmus maximus.
- Source: Ardtoe Hatchery.
- Loading density (fish/litre): 0.878.
- Feeding during test: no.
ACCLIMATION
- Acclimation period: 24 days.
- Acclimation conditions: Scophthalmus maximus were held under flow-through conditions in 200 litre capacity polythene tanks, containing 1 µm filtered ultra-violet treated seawater and supplied with gentle aeration.
- Type and amount of food: fed food in pellet form ad libitum.
- Feeding frequency: twice daily.
- Health during acclimation (any mortality observed): 0% mortality. - Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- no data
- Test temperature:
- 14.3-14.8°C
- pH:
- 7.65-7.99
- Dissolved oxygen:
- 89-97%
- Salinity:
- 37-38 ‰
- Nominal and measured concentrations:
- nominal concentration: 1.69 mg/l
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Material, size, headspace, fill volume: 30 litre capacity moulded soda-lime glass tanks.
- Aeration: gentle aeration.
- Renewal rate of test solution: at 48h.
- No. of organisms per vessel: 7.
- No. of vessels per concentration (replicates): 1.
- No. of vessels per control (replicates): 1.
- Biomass loading rate: 0.878 g/L.
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: natural local seawater pumped to holding tanks from Scapa Flow, Orkney.
- Treatment process: sand filtered to remove particulate, filtered to 1 µm, heated to 16°C, UV treated, degassed and held at a quantity of 3000 to 5000 litres.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no.
- Photoperiod: constant illumination. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1.69 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks:
- no mortality observed at the limit test concentration
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: measured at the end of the test (see table 2 above)
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: -
- Total alive fish afetr 96h exposure to teste material: 7/7
- The following validity criteria are fulfilled:
* Mortality of controls is less than 10% at the end of the test
* Test conditions (pH, temperature, salinity) were maintained during the test
* The oxygen saturation was maintained during the test and was above 60% of oxygen saturation
* There is a lack of information of data relating to the analytical monitoring showing that the concentrations of the test item were maintained during the test. However, the test has been performed under semi-static conditions and according to the long-term toxicity test to Daphnia on a very similar substance, the substance is stable during 48 hours. Thus, we can consider that in a short-term study, the substance is also stable.
For details see table 1 in ‘Any other information on results incl. tables’ - Sublethal observations / clinical signs:
Table 1: Summary of guideline criteria and values during test
Parameter
Guideline criterion
Observed values
Control pH over 96h
6-9
7.66 – 8.00
pH over 96h
6-9
7.65 – 7.99
Temperature (°C)
15 ±1.5
14.3 – 14.8
Dissolved oxygen (%)
≥60
89 – 97
Salinity (‰)
36±4
37 – 38
Control mortality (%)
≤10
0
Maximum loading of fish/litre
1g
0.878g
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item exhibited no adverse effects on turbot at the nominal concentration of 1.69 mg/L (corresponding to 0.51 mg active content/L).
- Executive summary:
The short-term aquatic toxicity of the REACh substance (2-Hydroxy-3-sulphopropyl) dimethyl[3-[(1-oxododecyl) amino]propyl]ammonium hydroxide (EC 242-893-1) was investigated in a GLP-compliant study performed with the marine fish species Scophthalmus maximus (turbot) in accordance with OSPARCOM guidelines. The test item exhibited no adverse effects on turbot at the nominal concentration of 1.69 mg/L (limit test) and the 96h-LC50 value is reported to be greater than 1.69 mg/L (corresponding to 0.51 mg active content/L).
Referenceopen allclose all
Description of key information
The short-term aquatic toxicity of the REACh substance (2-Hydroxy-3-sulphopropyl) dimethyl[3-[(1-oxododecyl) amino]propyl]ammonium hydroxide (EC 242-893-1) was investigated in a GLP-compliant study performed with the freshwater fish species Danio rerio (zebra fish) in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test) and EU Method C.1 (Höger, 2013). The test item exhibited no acute toxic effects on zebra fish at the nominal concentration of 28.1 mg/L (limit test) and the 96h-LC50 value is reported to be greater than 28.1 mg/L (corresponding to 12.9 mg solid content/L and 10 mg active content/L).
The short-term aquatic toxicity of the REACh subsance was otherwise investigated in two GLP-compliant studies performed with the marine fish species Scophthalmus maximus (turbot) in accordance with OSPARCOM guidelines (Richardson, 2009; Sacker, 2009). The 96h-LC50 is reported to be greater than 1.69 mg/L (corresponding to 0.51 mg active content/L) and 10 mg/L (corresponding to 4.8 mg solid content/L), respectively.
NB. In case the solid content of the test item was not reported in a study report, ECx values were expressed in active content. If relevant, these values were used for the chemical safety assessment (worst-case values).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 12.9 mg/L
Marine water fish
Marine water fish
- Effect concentration:
- 4.8 mg/L
Additional information
The short-term toxicity to fish of the substance EC 242-893-1 was investigated in three GLP-compliant studies (Höger, 2013; Richardson, 2009; Sacker, 2009) performed in accordance with standard methods, with no or minor deviations. These studies are considered as reliable (Klimisch 1 - with analytical monitoring - or Klimisch 2 - without analytical monitoring) and were selected as key studies for the endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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