Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 212-133-3 | CAS number: 764-99-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
in vitro
Ames test
The test item was tested for mutagenicity in the Ames test and in the E. coli- reverse mutation assay both in the standard plate test and in the preincubation test with and without the addition of a metabolizing system obtained from rat liver (S-9 mix) using the Salmonella strains TA 1535, TA 100, TA 1537, TA 98 and Escherichia coli WP2 uvrA at a dose of 20-5000 µg/plate (GLP guideline study, BASF, 1998). Positive control substances caused increases in the number of revertants as expected, indicating proper test conditions. The test substance showed no mutagenic effects at any dose level including the recommended limit dose of 5000 µg/plate.
Chromosome aberration test
The test item was assessed for its potential to induce structural chromosome aberrations in primary lymphocytes in vitro in two independent experiments at doses of 125 - 1580 µg/ml with and without metabolic activation (GLP guideline study, BioReliance, 2007). Under the experimental conditions reported no increased aberration frequencies were observed by the chromosome aberration test. Based on the findings of this in vitro mammalian chromosome aberration test using human peripheral blood lymphocytes, the test item was negative for the induction of structural or numerical chromosome aberrations in both the non-activated and the S9-activated test systems.
HPRT (read across, for justification see repeated dose toxicity)
The structural analogue 3,6,9,12-Tetraoxatetradeca-1,13-diene (CAS 765-12-8) of the test item was tested with and without metabolic activation for mammalian gene mutation (HGPRT locus) in Chinese hamster ovary cells (GLP guideline study, BASF SE, 2011). The concentration range tested was up to 2100 µg/mL (without and with metabolic activation by S9-Mix). No induction of forward mutations was observed in any of the experiments. Positive and negative controls gave the expected results. Therefore, the structural analogue of the test item is non-mutagenic in CHO cells (HGPRT-/+).
in vivo
Mouse micronucleus test
In a GLP guideline study according to OECD 474 (BioReliance, 2007), the test item was tested for clastogenicity and for the ability to induce spindle poison effects in ICR mice using the micronucleus test method. The test substance, dissolved in corn oil was administered by gavage to male and female animals at dose levels of 500 mg/kg, 1000 mg/kg and 2000 mg/kg bw body weight in a volume of 20 mL/kg body weight. As a negative control, corn oil (the selected vehicle) was administered to male mice by the same route, and gave frequencies of micronucleated polychromatic erythrocytes within the historical control range. The test item reduced the ratio of polychromatic erythrocytes to total erythrocytes relative to the respective vehicle controls in both sexes. Although these reductions did not occur in a dose-related manner, they suggest that the test item was bioavailable to the bone marrow target tissue. The test item did not produce a significant increase in the incidence of micronucleated polychromatic erythrocytes in either sex of any group relative to the respective vehicle control groups at either 24 or 48 hours after dose administration. Based on the observations in this study and under the conditions described in this report, the test item was not clastogenic in the mouse micronucleus assay.
Justification for selection of genetic toxicity endpoint
No study was selected since all studies were negative.
Short description of key information:
The test substance was not mutagenic in a bacterial reverse mutation assay in Salmonella and E.coli (OECD 471, Ames). Also, no mutagnic effects were observed in an in vitro chromosome aberration test using primary human blood lymphocytes and in an in vivo mouse micronucleus test after oral administration. In addition the read across substance 3,6,9,12-Tetraoxatetradeca-1,13-diene (CAS 765-12-8) showed no mutagenic effects in a gene mutation test in vitro (HPRT).
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Classification is not warranted according to the criteria of EU Directive 67/548/EEC and EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live1