1,1'-[oxybis(ethyleneoxy)]diethylene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-01-06 to 2007-01-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study with minor deviations (details e.g. for housing conditions were not provided).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

adopted December 17, 2001.

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.1100 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,1'-[oxybis(ethyleneoxy)]diethylene

EC Number:

212-133-3

EC Name:

1,1'-[oxybis(ethyleneoxy)]diethylene

Cas Number:

764-99-8

Molecular formula:

C8H14O3

IUPAC Name:

{2-[2-(ethenyloxy)ethoxy]ethoxy}ethene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ace Animals, Boyertown, PA
- Age at study initiation: approx. 9 weeks
- Weight at study initiation: Body weight range in males was 238 - 274 grams and in females was 172 - 206 grams
- Fasting period before study: 16 - 20 h
- Housing:The animals were identified by cage notation and indelible body marks, and housed in suspended wire mesh cages; 1/cage. Bedding was placed beneath the cages and changed at least three times/week.
- Diet: Fresh PMI Rodent Chow (Diet #5021) was freely available
- Water: Ad libitum
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): Temperature was controlled
- Photoperiod (hrs dark / hrs light): 12 hour light/dark cycle

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 0.55 mL

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female rats

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were observed at 1, 2 and 4 hours postdose and once daily thereafter for 14 days for toxicity and pharmacological effects. All animals were observed twice daily for mortality. Body weights were recorded immediately pretest, weekly and at termination.
- Necropsy of survivors performed: All animals were examined for gross pathology.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

All ten animals survived the 2000 mg/kg oral dose

Clinical signs:

other: Instances of chromorhinorrhea, lethargy, ataxia, hunched posture, few feces, bloated abdomen, chromodacryorrhea, soiling of the anogenital area and/or emaciation ware noted in several rats during the study. Three rats were normal throughout the entire ob

Gross pathology:

The following abnormalities were noted in one or two rats at necropsy: slight or scattered red areas on the thymus, red areas on the intestines that ranged from slight or scattered to moderate or few; and/or slight or scattered soiling of the anogenital area. Bifurcated spleen was noted in two rats, although this abnormality should not be considered to be a result of treatment with the test article.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: OECD GHS