D-Glucopyranoside, methyl, mixed decanoates and octanoates and oleates

Toxicity to aquatic algae and cyanobacteria

Description of key information

The 72-h acute toxicity of the test item to Pseudokirchneriella subcapitata was studied in a GLP study performed according to OECD TG 201 and EC Method C.3. The ErC50 (0-72h) was > 100 mg/L and the NOErC was 10 mg/L, based on nominal concentrations.

Key value for chemical safety assessment

EC10 or NOEC for freshwater algae:

10 mg/L

Additional information

One study is available. In this study, performed under GLP according to OECD TG 201 and EC Method C.3, Pseudokirchneriella subcapitata was exposed to an aqueous dispersion of the test item at concentrations of 1.0, 3.2, 10, 32 and 100 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 °C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group using a haemocytometer and light microscope. Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.27 to 41 mg/L. A decline in measured test concentration was observed after each 24 hour interval in the range of 0.0082 to 32 mg/L after 24 hours, less than the limit of quantification (LOQ), determined to be 0.0059 mg/L to 1.4 mg/L after 48 hours and less than the LOQ to 0.38 mg/L after 72 hours. As the test item concentrations were calculated by monitoring and analyzing for only one component of the test item, the low percentage nominal values could be attributed to the solubility/stability of that single component and not the test item as a whole. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal concentrations only.The 72 -h ErC50 was > 100 mg/L and the NOErC was 10 mg/L.