6-[methyl(phenylsulphonyl)amino]hexanoic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984-08-27 to 1984-09-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according the OECD Guideline 203 with no deficiencies

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6-[methyl(phenylsulphonyl)amino]hexanoic acid

EC Number:

256-289-0

EC Name:

6-[methyl(phenylsulphonyl)amino]hexanoic acid

Cas Number:

46948-72-5

Molecular formula:

C13H19NO4S

IUPAC Name:

6-(N-methylbenzenesulfonamido)hexanoic acid

Details on test material:

n.a.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
n.a.

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

n.a.

Test solutions

Vehicle:

no

Details on test solutions:

The test was separated two allow a fine tuning of the test concentrations (all nominal). Part 1: 0 and 500 mg/L, Part 2: 0, 10 and 100 mg/L, Part 3: 0, 177, 250 and 354 mg/L. Reconstituted water. Solid test substance was dispersed in the test water with Ultra-Turrax (highest speed) and afterwards stired for 2 hours. Test concentrations 10 to 354 mg/L were visually clear. At 500 mg/L solids at the bottom of the test container was observed.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Own breeding at Hoechst laboratory, hatched 1984-06-13 respectively 1984-06-06. Length of fish 3.2 cm (mean). 10 fish per test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Remarks on exposure duration:

h

Post exposure observation period:

n.a.

Test conditions

Test temperature:

22° C +/- 1

pH:

7.9-8.1

Dissolved oxygen:

80% saturation

Salinity:

n.a.

Nominal and measured concentrations:

nominal concentration

Details on test conditions:

O2: 80% saturation, 12h light period, fish density < 1 g fish/L, feed: 2 per day ad libitum (Tetra min fish feed). Fish were visually inspected after 2, 4, 24, 48, 72 and 96h.

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

177 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

210 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: The LC50 value was derived as geometric mean of LC0 (96h) and LC100 (96h) being adjacent concentrations as no statistical evaluation was possible.

Details on results:

Mortality rates were recorded after 48 and 96h.

Results with reference substance (positive control):

n.a.

Reported statistics and error estimates:

A statistical evaluation was not possible as the LC0 and LC100 were adjacent concentrations. Instead the geometric mean of LC0 and LC100 was used to derive the LC50.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The registration substance was tested in an acute fish test according OECD Guideline 203. The LC50 (96h) of 210 mg/L was derived as geometric mean of the two adjacent concentrations reflecting the LC0 (96h) of 177 mg/l and the LC100 (96h) of 250 mg/L).