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EC number: 248-394-5 | CAS number: 27310-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted following OECD guideline, but report does not mention whether GLP was followed and sufficient data is available for interpretation of results.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- OECD guideline 202 followed.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 7-aminonaphthalene-1,3,5-trisulphonic acid
- EC Number:
- 248-394-5
- EC Name:
- 7-aminonaphthalene-1,3,5-trisulphonic acid
- Cas Number:
- 27310-25-4
- Molecular formula:
- C10H9NO9S3
- IUPAC Name:
- 7-aminonaphthalene-1,3,5-trisulfonic acid
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): FAT 93527/A
- Physical state: Powder
- Analytical purity: ca. 50%
- Lot/batch No.: Lot 0031026A0
- Expiration date of the lot/batch: January 10, 2006
- Storage condition of test material: room temperature
- Solubility: ca.120g/L
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- None
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Stock solution: 100.0 mg test item were mixed with 200 mL test medium in a volumetric flask, made up to 1000 mL with test water.
Test concentrations: A range-finding test with the following test concentrations was carried out: 0.01, 0.1, 1.0, 10 and 100 mg/L No Immobilisation of Daphnia were observed at the highest concentration, hence the test concentrations for the definitive test were chosen as: 4.3, 9.4, 21, 45 and 100 mg/L
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Solvias AG, Switzerland
- Age at study initiation (mean and range, SD): 24 hr
- Method of breeding: The Daphnia were bred in the laboratories of the GLP Test Facility Solvias under standardised conditions.
BREEDING:
Cultures of Daphnia magna are held in glass vessels containing approximately 3 litres of reconstituted water at 18 -22°C. The water is renewed partially three times per
week. Daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplementpd by a Tetramin-extract in such quantities that the food is consumed within 24 hours.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- Study terminated after 48 h.
Test conditions
- Hardness:
- 178mg CaCO3/L
- Test temperature:
- 20.2 - 20.8 Deg C
- pH:
- 8.1
- Dissolved oxygen:
- 96 - 97%
- Salinity:
- No data
- Nominal and measured concentrations:
- 4.3, 9.4 and 21, 45, and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml beaker
- Type (delete if not applicable): open
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 50-100 ml test medium with 10 animals per beaker
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
NaHCO3: 65mg/L
MgSO4*7H2O: 123.3mg/L
KCl: 5.8mg/L
CaCI2 *2H2O: 294mg/L
Na2SiO3: 4.3mg/L
NaNO3: 274 micro g/L
KH2PO4: 143 micro g/L
K2HPO4: 184 micro g/L
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4 : 1
- Na/K ratio: 10 : 1
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light daily, 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS:
- Range finding study: 0.01, 0.1, 1.0, 10 and 100 mg/L
- Test concentrations: 4.3, 9.4, 21, 45 and 100 mg/L
- Results used to determine the conditions for the definitive study: Immobilisation - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No mortality
- Results with reference substance (positive control):
- - EC50/LC50: The EC50 for potassium dichromate after 24 hours of exposure was determined among 46 laboratories in an international ring-test with Daphnia magna and found to be at 1.5 mg/L (0.9 to 1.9 mg/L).
Therefore, the cultures of Daphnia magna used by the test facility were demonstrated to be adequately sensitive. - Reported statistics and error estimates:
- No statistics used.
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value after 48 hours was determined to be greater than 100 mg/L
The EC100 value after 48 hours was determined to be greater than 100 mg/L - Executive summary:
FAT 93527/A was tested for its acute toxicity to Daphnia magna in a 48 hour static test following OECD test guideline 202.
The nominal concentrations of FAT 93527/A tested were: 4.3, 9.4, 21, 45 and 100 test item/L. Additionally, a blank control without test item was carried out. Test concentrations were prepared from a stock solution.
No Daphnia were immobilised in the control and test concentrations of 4.3, 9.4 and 21, 45, and 100 mg/L after an exposure period of 48h.
The EC50 value after 48 hours was determined to be greater than 100 mg/L
The EC100 value after 48 hours was determined to be greater than 100 mg/L.
According to Directive 93/21/EEC (adaptation to Directive 67/548/EEC) the ecotoxicological classification of FAT93527/A is 'not toxic' to Daphnia magna following an exposure period of 48 hours.
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