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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

in vitro gene mutation study in bacteria
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not documented
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference Type:
Mutagenicity of alkyl glycidyl ethers in three short-term assays
Thompson, E.D. et al.
Bibliographic source:
Mutat. Res. 90, 213-231 (1981)

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
Method: other. Bacterial reverse mutation conducted according to method of Ames
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Reference substance name:
alkyl glycidyl ethers
alkyl glycidyl ethers
Details on test material:
Test substance: other TS - alkyl glycidyl ethers
Purity: 97.7 %, free from epichlorohydrin


Target gene:
Not documented
Species / strain
Species / strain / cell type:
other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
Details on mammalian cell type (if applicable):
Not applicable
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
No information provided
Test concentrations with justification for top dose:
20.6/ 61.7/ 185.2/ 555.5/ 1667/ 5000 ug/plate
Vehicle / solvent:
Not documented
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
Type: Ames test - bacterial test system - various strains of S.typhimurium
Evaluation criteria:
Not documented
Not documented

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No additional information provided
Remarks on result:
other: other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
Migrated from field 'Test system'.

Any other information on results incl. tables

Both with/ without activation, dose-dependent increase in mutation frequency of TA 100 and TA 1535,

no increase with TA 98 and TA 1537, and TA 1538.

Applicant's summary and conclusion

Interpretation of results (migrated information):
positive with and without metabolic activation

In this bacterial reverse mutation assay with and without metabolic activation glycidol showed positive results.
Executive summary:

In the study conducted by Thompson (1981), the test material, alkyl glycidyl ethers, were examined for their ability to cause genetic mutations when tested in strains of Salmonella typhimurium, specifically TA 98, TA 100, TA 1535, TA 1537 and TA 1538, in the presence and absence of metabolic activation. The test substance was tested at concentrations of 20.6, 61.7, 185.2, 555.5, 1667 and 5000 ug/plate. Based on the results of this study, the test substance induced a positive result when tested with and without metabolic activation.

Under the conditions of the study and based on the results, the test substance should be considered to be a genotoxicity Category 2 mutagen and should have the signal word Warning and the hazard statement H341: Suspected of causing genetic defects, in accordance with Regulation EC No. 1272/2008. According to Directive 67/548/EEC, the test substance should be classified as a Category 3 mutagen and should have the xymbol Xn and the risk phrase R68 Possible risk of irreversible effects associated with it.