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Administrative data

Description of key information

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

A local lymph node assay was conducted to assess the potential of Glycidol to cause skin sensitisation in the mouse.

Following a preliminary screening test using the undiluted test article, the main study was conducted using the undiluted test article and concentrations of 25 and 50% v/v in dimethylformamide. Groups of four female CBA / CaCrl mice were subjected to topical applications of vehicle or of one of the test formulations to the outer aspect of the auditory pinnae once daily on Days 1, 2 and 3.

On Day 6 a 20 μCi dose of tritiated3H-methyl thymidine was injected intravenously into each mouse. Five hours later the auricular lymph nodes were recovered from each animal. The nodes from mice subjected to the same treatment were pooled and suspensions of the cellular components of the lymph nodes were prepared in 5% w/v trichloroacetic acid and processed through a scintillation counter.

Test results are expressed in terms of Stimulation Indices, the ratios of the mean scintillation count per group obtained from the test groups relative to the corresponding mean scintillation count from controls. The threshold level for the Stimulation Index to be considered a positive indicator of the potential to cause skin sensitisation is 3.0.The Local Lymph Node Assay demonstrated that Glycidol does not have the potential to cause skin sensitisation.The test article did not meet the criteria for classification as a sensitiser according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS).

Migrated from Short description of key information:
The Local Lymph Node Assay demonstrated that Glycidol does not have the potential to cause skin sensitisation.
The test article did not meet the criteria for classification as a sensitiser according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS).

Justification for classification or non-classification

The LLNA SI value for all three tested groups was less than the classification threshold of 3.0