Registration Dossier
Registration Dossier
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EC number: 202-045-3 | CAS number: 91-16-7
Biodegradation in soil
Administrative data
- Endpoint:
- biodegradation in soil, other
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Paper presents description of test method and results of degradation step by nitrification. However, not adequate for the purpose of hazard/risk assessment or hazard classification.
Data source
Reference
- Reference Type:
- publication
- Title:
- Biodegradation of aromatic ethers by nitrifying bacteria
- Author:
- Chang, S.W.
- Year:
- 1 999
- Bibliographic source:
- International in situ and on-site bioremediation symposium, 5th, San Diego (7), pp. 131-135
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Cells from a culture of the soil nitrifying bacterium Nitrosomonas europaea were tested for their capability to degrade the test substance in a batch experiment. Die-away of parent and formation of oxidation products in time was detected by HPLC
- GLP compliance:
- not specified
- Test type:
- laboratory
Test material
- Reference substance name:
- Veratrole
- EC Number:
- 202-045-3
- EC Name:
- Veratrole
- Cas Number:
- 91-16-7
- Molecular formula:
- C8H10O2
- IUPAC Name:
- 1,2-dimethoxybenzene
- Test material form:
- other: liquid
- Details on test material:
- no data
Constituent 1
- Radiolabelling:
- no
Study design
- Oxygen conditions:
- aerobic
- Soil classification:
- other: not tested in soil; Cell culture of one species
- Details on soil characteristics:
- not applicable
- Parameter followed for biodegradation estimation:
- other: analysis of test material and degradation products
- Details on experimental conditions:
- Nitrosomonas europaea culture in phosphate buffer, 30 degree C
Results and discussion
- Transformation products:
- yes
Identity of transformation productsopen allclose all
- No.:
- #1
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- 2-methoxyphenol
- Inventory number:
- InventoryMultipleMappingImpl [inventoryEntryValue=EC 201-964-7]
- CAS number:
- 90-05-1
- Identity:
- Phenol, 2-methoxy-
- Molecular formula:
- C7H8O2
- Molecular weight:
- 124.137
- SMILES notation:
- COc1ccccc1O
- InChl:
- InChI=1/C7H8O2/c1-9-7-5-3-2-4-6(7)8/h2-5,8H,1H3
- No.:
- #2
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- catechol
- Identifier:
- common name
- Identity:
- catechol
- Details on transformation products:
- no further details
- Evaporation of parent compound:
- not specified
- Volatile metabolites:
- not specified
- Residues:
- not specified
- Details on results:
- In general, degradation rates decrease when the number of methoxyl groups on a benzene molecule increase. Therefore veratrole degrades slower than anisole.
- Results with reference substance:
- no data
Any other information on results incl. tables
Reaction is catalyzed by the non-specific ammonia-oxidizing enzyme ammonia monooxygenase.
Applicant's summary and conclusion
- Executive summary:
The test substance is degraded by Nitrosomonas europaea. Transformation products are 2 -methoxyphenol and catechol. The process is catalysed by the presence of the non-specific enzyme ammonia mono-oxygenase (AMO).
The test is performed with a culture of one specific bacterium and does not represent a system or data that can be used to derive degradation rates relevant for the environment. Thus the study is not adequate for hazard/risk assessment or classification.
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