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EC number: 939-505-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented GLP study according to international guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- Deviations:
- yes
- Remarks:
- NH4Cl was omitted from the medium to prevent oxygen consumption due to nitrification
- Qualifier:
- according to guideline
- Guideline:
- other: ISO Test Guidelines 10107 (1994)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Certifcate dates from 2010. The test substance data were submitted by the sponsor (unless stated otherwise), who accepted full responsibility for the validity of these data.
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water
- Details on inoculum:
- - Source of inoculum: River water was sampled from the Rhine near Heveadorp, the Netherlands (19-
01-2012). The nearest plant (Arnhem-Zuid) treating domestic wastewater
biologically was 3 km upstream.
- Preparation of inoculum for exposure: The river water was aerated for 7 days before
use to reduce the endogenous respiration. River
water without particles was used as inoculum. The particles were removed by
sedimentation after 1 day while moderately aerating. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 1 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The river water used in the Closed Bottle test was spiked per liter of water with
8.5 mg KH2PO4, 21.75 mg K2HPO4, 33.3 mg Na2 HPO4·2H2O, 22.5 mg
MgSO4·7H2O, 27.5 mg CaCl2, 0.25 mg FeCl3·6H2O. Ammonium chloride was
omitted from the medium to prevent nitrification.
TEST SYSTEM
- Culturing apparatus: 0.30 L BOD (biological oxygen demand) bottles with glass stoppers
- Number of culture flasks/concentration: 10 for test substance, 6 for sodium acetate
- Measuring equipment: The dissolved oxygen concentrations were determined electronically using an oxygen electrode (WTW TrioXmatic EO 200) and meter (WTW OXI 530) (Retsch, Ochten, the Netherlands). The pH was measured using a Eutech Cyberscan pH11 pH meter (Eutech Instruments, Nijkerk, the Netherlands). The temperature was measured and recorded with a sensor connected to a data logger.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- acetic acid, sodium salt
- Remarks:
- concentration in the bottle: 6.7 mg/L; source: Acros organics
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 13
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 83
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 83
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Fyrquel EHC PLUS (low TPP) did not cause a reduction in the endogenous respiration. The test substance is therefore considered to be non-inhibitory to the inoculum. Fyrquel EHC PLUS (low TPP) was biodegraded by 83% at day 28 in the closed bottle test. Hence, this substance should be classified as readily biodegradable.
The test is valid as shown by an endogenous respiration of 1.4 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded by 90 of its theoretical oxygen demand after 14 days. Finally, the most important criterion was met by oxygen concentrations > 0.5 mg/L in all bottles during the test period. - Executive summary:
Theoretical oxygen demand (ThOD)
The calculated theoretical oxygen demand (ThOD) of Fyrquel EHC PLUS (low TPP)
is 2.3 mg/mg. The ThOD of sodium acetate is 0.78 mg/mg.
Toxicity
Inhibition of the degradation of a well-degradable compound, e.g. sodium acetate
by the test substance in the Closed Bottle test was not determined because
possible toxicity of Fyrquel EHC PLUS (low TPP) to microorganisms degrading
acetate is not relevant. Inhibition of the endogenous respiration of the inoculum
by the test substance at day 7 was not detected. The non toxicity was deduced
from the oxygen consumption of 0.1 mg/L at day 7. Therefore, no
inhibition of the biodegradation due to the "high" initial test substance
concentration of 1.0 mg/L is expected. The initial test substance concentration is
high compared to environmental concentrations of organic substances (low μg/L
range).
Test conditions
The pH of the media was 8.0 at the start of the test. The pH of the medium at day
28 was 8.1 (control), 8.0 (controls), and 7.9 (test). Temperatures were within the
prescribed temperature range of 22 to 24°C.
Validity of the test
The validity of the test is demonstrated by an endogenous respiration of
1.4 mg/L at day 28. Furthermore, the differences of the replicate values
at day 28 were less than 20%. The biodegradation percentage of the reference
compound, sodium acetate, at day 14 was 90. Finally, the validity of the test is
shown by oxygen concentrations > 0.5 mg/L in all bottles during the test period.
Biodegradability
Fyrquel EHC PLUS (low TPP) is biodegraded by 83% at day 28 in the Closed Bottle
test (Figure and Tables I-II). The pass level of 60% was reached after 8 days
upon achieving 10% biodegradation. Fyrquel EHC PLUS (low TPP) therefore
fulfilled the 10-day time window criterion. Fyrquel EHC PLUS (low TPP) is
therefore classified as readily biodegradable based on the biodegradation
percentage of 83% at day 28 and the time window of 8 days.
The pass level in ready biodegradability tests using either oxygen consumption or
carbon dioxide evolution is ≥ 60% at day 28. Biodegradation percentages ≥ 60 are interpreted as complete (ultimate) biodegradation. Biodegradation percentages ranging from 60 to ~95% (≥ 100% biodegradation is impossible) are found for all utimately biodegradable substances. The biodegradation percentage of 83% reached at day 21 therefore shows that the biodegradation of Fyrquel EHC PLUS (low TPP) was completed at day 21.
Reference
Description of key information
Two studies performed according to the OECD 301D guideline are available.
After 28 days, the biodegradability was 78% and 83% for the multi-constituents containing respectively 4% and 2% TPP.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Two ready biodegradability tests in which biodegradability was measured in an aerobic aqueous medium were performed, one study with Fyrquel EHC PLUS (van Ginkel 2012a) and another study with Fyrquel EHC PLUS (low TPP) (van Ginkel 2012b) as test substance. The ready biodegradability test was performed in the closed bottle test according to OECD 301 D guideline.
Both test substances did not cause a reduction in the endogenous respiration and are therefore considered to be non-inhibitory to the inoculum. Fyrquel EHC PLUS and Fyrquel EHC PLUS (low TPP) were respectively biodegraded by 78% and 83% at day 28 in the closed bottle test and the validity criteria were fulfilled. Hence, both substances should be classified as readily biodegradable.
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