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EC number: 627-083-1 | CAS number: 244235-47-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
- Version / remarks:
- 29 July 2016
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 19.04.2017
- Expiration date of the lot/batch: 19 Apr 2022
- Purity test date: 100% (as UVCB) - Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The test substance C9-Aldoxime is liquid and poorly soluble in water. As the test substance is soluble in acetone, a stock solution in acetone was prepared. The amount of 25000.4 mg test substance was weighed into a 50 mL volumetric flask. The test substance was dissolved by addition of acetone as solvent. The solution was filled up to the mark afterwards. The test substance was completely dissolved. The solution was clear and yellowish.
Aliquots of the stock solution with required amounts of test substance for each scheduled test concentration were carefully mixed with quartz sand. The organic solvent was removed by evaporation and subsequently blended with prepared test substrate. Thereafter, the water content of the soil was adjusted to about 50% WHCmax using deionized water and was mixed once again. The test mixtures were distributed to the test units. - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: in-house breeding
- Age at test initiation (mean and range, SD): 9 months
- Weight at test initiation (mean and range, SD): 300 - 600 mg
ACCLIMATION
- Acclimation period/conditions: Before starting the test, the worms selected were acclimatized in the test substrate in a plastic box under test conditions through overnight under permanent light and without feeding. For this, a plastic box was filled up with approx. 750 g test substrate (Dw) and added with a sufficient number of adult worms that were sorted out from the breeding boxes. - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 28 d
- Test temperature:
- 20.5 ° C – 21.8 °C (exception on 22nd – 27th days of exposure with ≤ 0.5 °C increase in measurement due to technical reasons; with 22.5 ° C on 22nd day and 22.4 °C on 27th day of exposure)
- pH:
- 5.7 - 5.9 (start of exposure)
6.8 - 7.0 (end of exposure) - Moisture:
- 50 ±10 % WHCmax (mean value)
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Plastic dishes with a volume of about 1.0 L, dimensions 11×15.5×6 [cm] with transparent and punctured lid
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 8
OTHER TEST CONDITIONS
- Photoperiod: 16h light: 8h dark
- light intensity: 605 Lux, (mean of 5 single measurements: 769, 641, 429, 673 and 513 lux)
EFFECT PARAMETERS MEASURED: Mortality, weight and reproductive output was recorded throughout the test period of 28 days.
VEHICLE CONTROL PERFORMED: yes - Nominal and measured concentrations:
- Nominal: 62.5, 125, 250, 500 and 1000 mg/kg
- Reference substance (positive control):
- yes
- Remarks:
- Carbendazim (BAS 346 F)
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 94 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass change
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: no
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 28d-EC50 = 1.19 mg/kg dw - Validity criteria fulfilled:
- yes
- Conclusions:
- The 28d-EC10 was determined to be 94 mg/kg dw.
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- see Analogue justification document in chapter 13
- Reason / purpose for cross-reference:
- read-across source
- GLP compliance:
- yes (incl. QA statement)
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 94 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
Referenceopen allclose all
Description of key information
EC10(28d) = 94 mg/kg dw (nominal) for reproduction of Eisenia fetida (OECD 222, read across)
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 94 mg/kg soil dw
Additional information
Since no study assessing the long-term toxicity of Ethanone, 1-(2-hydroxy-5-nonylphenyl)-, oxime, branched (CAS 244235-47-0) to soilamcroorganisms is available, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 Grouping of substances, a read-across to the structurally very similar source substance Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched (CAS 174333-80-3), was conducted. The only structural difference between the source substance and the main component of the target substance is the lack of a methyl group at the oxime carbon of the source substance. The read across is justified due to (i) the similarity of structure and functional groups and accordingly (ii) similar physico-chemical properties resulting in a similar environmental fate and ecotoxicity profile (see table below).
Substance |
Ethanone, 1-(2-hydroxy-5-nonylphenyl)-, oxime, branched |
Benzaldehyde, 2-hydroxy-5-nonyl, oxime, branched |
CAS number |
244235-47-0 |
174333-80-3 |
Structure |
see attachment |
see attachment |
Molecular formula |
C17H27NO2 |
C16O2NH25 |
Molecular weight |
~ 277 g/mole |
~ 263 g/mole |
PC parameter |
|
|
Water solubility |
> 0.02 < 0.1 mg/L (EU method A.6) |
0.4 mg/L (EU method A.6) |
Partition coefficient |
> 5.7 (EU method A.8) |
5.5 (EU method A.8) |
Vapour pressure |
< 1.5 Pa at 20 °C (OECD 104) |
0.37 Pa at 20 °C (OECD 104) |
Environmental fate |
|
|
Biodegradability |
1 % in 28 days (BODIS) |
0 % in 28 days (OECD 302c) |
Adsorption [log KOC] |
3.9 (OECD 121) |
3.7 (OECD 121) |
Hydrolysis |
not relevant due to very low water solubility |
|
Ecotoxicology |
|
|
Short-term toxicity to fish [96h-LC50] |
0.46 mg/L (EU method C.1) |
1.1 mg/L (EU method C.1) |
Short-term toxicity to aquatic invertebrates [48h-EC50] |
- |
2.7 mg/L (EU method C.2) |
Long-term toxicity to aquatic invertebrates [21d-NOEC] |
2.8 mg/L (OECD 211) |
0.189 mg/L (OECD 211) |
Short-term toxicity to algae [72h-EC50] |
760 mg/L (OECD 201) |
36.3 mg/L (OECD 201) |
Long-term toxicity to algae [72h-NOEC] |
472 mg/L (OECD 201) |
14.9 mg/L (OECD 201) |
Toxicity to microorganisms [3h-EC10] |
260.1mg/L (OECD 209) |
200.4 mg/L (OECD 209) |
The study with the source substance was performed according to OECD guideline 222 under GLP conditions using Eisenia fetida as test organism (BASF 2018). Acetone was used as solvent to a prepare stock solution of the test substance. The organic solvent was removed by evaporation and subsequently blended with prepared test substrate. Nominal concentrations between 62.5 and 1000 mg/kg dw were tested for effects on survival, weight and reproductive output throughout a test period of 28 days. No mortality was observed in the controls and any treatment. The 28d-NOEC value for reproduction was determined to be 125 mg/kg dw. The 28d-EC10 for reproduction is determined to be 94 mg/kg dw.
Additional data are available for Phenol, 4-nonyl-,branched, which is a secondary component and structurally similar to the main component of the substance. The only structural difference between the source substance and the target substance is the lack of an ketoxime group at the phenol ring of the molecule. The effect of increasing 4-nonylphenol (NP) concentrations (0, 10, 30, 90, and 270 mg/kg) in soil microcosms containing a simplified soil community was investigated over three sampling dates (28, 56, and 112 days) using the principal response curves method (Domene et al. 2010). The soil community did not change significantly at concentrations below 90 mg/kg, which was selected as the NOEC value. The highest concentration (270 mg/kg) changed the community significantly after 28 and 56 days, but this effect disappeared after 112 days.
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