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EC number: 209-935-0 | CAS number: 598-50-5
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,3-dimethylurea
- EC Number:
- 202-498-7
- EC Name:
- 1,3-dimethylurea
- Cas Number:
- 96-31-1
- IUPAC Name:
- 1,3-dimethylurea
- Details on test material:
- - Name of test material (as cited in study report):1,3-Dimethylurea
- Lot/batch No.: 00001075-0
- Analytical purity: 98.6 %
Water-solubility: 1000 g/l
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage; not adapted.
- Concentration of sludge: 30 mg/l - Duration of test (contact time):
- 21 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 90 - 100
- Sampling time:
- 21 d
- Details on results:
- Duration of the adaptation phase: 6 days.
Duration of the degradation phase: 11 days.
Degradation of the test substance at the end of the 10-day
Window: 90-100 % DOC removal.
Degradation of the test substance at the end of the test: 90-100 % DOC removal.
Physico-chemical (abiotic) elimination of the test
Substance: < 10 % at the end of the test.
Elimination of the test substance by adsorption: < 10 % after 5 days.
Degradation of the reference substance after 14 days: 90 -100 % DOC removal.
Degradation in the inhibition control after 14 days: 90 - 100 % DOC removal.
The validity criteria as laid down in OECD TG 301 were fulfilled.
Kinetic of test substance (in %):
0 after 1 day(s)
0 after 3 day(s)
= 0 -6 after 5 day(s)
= 23 - 25 after 7 day(s)
= 93 - 94 after 10 day(s)
Kinetic of control substance (in %):
= 9 after 1 day(s)
= 94 after 3 day(s)
Degradation products: not measured
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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