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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Retinol
EC Number:
200-683-7
EC Name:
Retinol
Cas Number:
68-26-8
Molecular formula:
C20H30O
IUPAC Name:
(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraen-1-ol
Details on test material:
- Name of test material (as cited in study report): Retinol crystalline
- Lot/batch No.: 090-04-06
- Analytical purity: 93.9 %

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge from laboratory wastewater plant treating municipal sewage. Concentration of the dry substance 1 g/l.
Preparation of the inoculum : The inoculum was washed and brought to a concentration of 5 g/I dry substance, then aerated during the night (24 h). 50 ml was added to the test solution total volume of 250 ml to obtain a concentration of 1 g dry substance/l in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min

Test conditions

Test temperature:
20 +/- 2 °C
Dissolved oxygen:
Oxygen concentration during aeration: > 2.5 mg/l
Oxygen concentration immediately before measurement: > 6.5 mg/l
Duration of the measurement of oxygen consumption: 8-10 min
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- Fill volume: 250 ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenole

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
Reference substance: EC50 = 27 mg/l

Applicant's summary and conclusion