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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

TK 12146, ACCELERATOR DY 9577 was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 (March 1991) and NEN-EN-ISO 10712 (1996).

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
10 000 mg/L

Additional information

TK 12146, ACCELERATOR DY 9577 was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 (March 1991) and NEN-EN-ISO 10712 (1996).

Suspensions of Pseudomonas putida were exposed to concentrations ranging from 312.5 to 10,000 mg TK 12146, ACCELERATOR DY 9577 per litre. After incubation the extinction values of the bacterial cell suspensions were measured. No toxicity was observed.

Based on the results of the test presently performed, the toxicity threshold value (EC10) of TK 12146, ACCELERATOR DY 9577 was over 10,000 mg/l.

The "Bewertungszahl" (Assessment figure or value for bacterial toxicity) is therefore < 1.9 (Bewertung wassergefahrdender Stoffe, 1989).

The EC50 value for the reference substance, 3,5-dichlorophenol, tested during the same experiment was 29 mg/l. Since this was within the accepted range of 10-30 mg/l for the EC50 and the multiplication factor of the bacteria within the test was over 60, it was concluded that the test conditions were optimal and the results obtained were valid.