Trichloro(N,N-dimethyloctylamine)boron

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 March 1996 to 11 April 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP methodology followed.

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

not specified

Remarks:

No information on GLP methodology was indicated.

Analytical monitoring:

yes

Details on test solutions:

Initial conc. 57 mg per 2 L (Bottle A)
57 mg per 2 L (Bottle A)

Parameter followed for biodegradation estimation
CO2 evolution

Details on inoculum:

Activated sludge freshly obtained from a municipal sewage treatment plant: "Waterschap de
Maaskant", s-Hertogenbosch, the Netherlands. The sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 5.7 g/L in the concentrated
sludge. This was outside the protocolled range (3-5 g/L). However, this was found to have had no effect on the outcome of the study. Before use, the sludge was allowed to settle for at least 30 min and the liquid decanted for use as inoculum at the amount of 10 mL/L of mineral medium.

Test type:

other: other: Activated sludge freshly obtained from a municipal sewage treatment plant.

Total exposure duration:

28 d

Reference substance (positive control):

yes

Remarks:

Sodium acetate

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

57 mg/L

Nominal / measured:

nominal

In the toxicity control more than 25% degradation occured in 14 days (based on ThCO2). Therefore, the test substance was assumed to be not inhibitory.

Monitoring of temperature and pH.

The temperature recorded during the study varied between 20 and 23°C, except for a few hours on day 22, when a deviation of 2°C from the temperature range mentioned in the protocol was noted, but was considered not to have affected the integrity of this study. The pH values of the different test media were:

	Just before the start of the test	On day 28
Blank control (A)	7.45	7.81
Blank control (B)	7.48	7.93
Positive control	7.49	8.17
TK 12146 (A)	7.48	7.63
TK 12146 (B)	7.49	7.60
Toxicity control	7.53	8.01

Conclusions:

Under study conditions, In the toxicity control more than 25% degradation occurred in 14 days (based on ThCO2). Therefore, the test substance was assumed to be not inhibitory and NOAEC value was found to be 28.5 mg/L

Executive summary:

A study was conducted to determine the biodegradation in water of the test substance according to OECD Guideline 301 B and EU Method C.4-C in CO2 evolution test (Modified Sturm test) without GLP. Trichloro(N,N-dimethyloctylamine) boron was tested at approximate concentration of 57 mg per 2 liters, corresponding to ca. 12 mg TC/L. The Total Carbon content (TC) of test substance was determined to be 42.2%. The Theorical CO2 production (ThCO2) of test substance was calculated to be 1.547 mg CO2/mg. Under study conditions, In the toxicity control more than 25% degradation occurred in 14 days (based on ThCO2). Therefore, the test substance was assumed to be not inhibitory and NOAEC value could be considered to be 28.5 mg/L (Desmares-Koopmans, 1996).

Description of key information

 

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

28.5 mg/L

Additional information

A study was conducted to determine the biodegradation in water of the test substance according to OECD Guideline 301 B and EU Method C.4-C in CO2 evolution test (Modified Sturm test) without GLP. Trichloro(N,N-dimethyloctylamine) boron was tested at approximate concentration of 57 mg per 2 liters, corresponding to ca. 12 mg TC/L. The Total Carbon content (TC) of test substance was determined to be 42.2%. The Theorical CO2 production (ThCO2) of test substance was calculated to be 1.547 mg CO2/mg. Under study conditions, In the toxicity control more than 25% degradation occurred in 14 days (based on ThCO2). Therefore, the test substance was assumed to be not inhibitory and NOAEC value could be considered to be 28.5 mg/L (Desmares-Koopmans, 1996).

A study was conducted to assess the toxicity of the test substance, trichloro(N,N-dimethyloctylamine)boron for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 and NEN-EN-ISO 10712. Suspensions of Pseudomonas putida were exposed to concentrations ranging from 312.5 to 10,000 mg test substance per liter. After incubation the extinction values of the bacterial cell suspensions were measured. No toxicity was observed. Under study conditions, the EC10 was determined to be 10,000 mg/L (Bewertung wassergefahrdender Stoffe, 1989).