Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29 April 1996 to 3 May 1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP methodology followed.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Species: Carp (Cyrinus carpio, Teleostei, cyprinidae) (Linnaeus, 1758)
- Source: Zodiac proeffac, "DeHaar Vissen", L.U. Wageningen, The Netherlands.
- Mean lenght: 2.4 +/- 0.25 cm
- Meam weight: 0.24 +/- 0.05 cm
- Characteristic: F1 from a single parent-pair bred in UV-treated water.
- Reason for selection: This system has been selected as an internationally accepted species.
- Quatantin/ Acclimation: At least 12 days after delivery
- Feeding: Daily with Trouvit

Validity of batch: In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
At 4, 24, 48, 72 and 96 hours following the start of exposure.
Hardness:
250 mg CaCO3 per liter
Test temperature:
20.5 to 20.6 °C
pH:
8.0
Dissolved oxygen:
Dissolved Oxygen (DO): 9.1 at day 0 and 8.4 at day 4.
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 0.1/ 1.0/ 10 and 100 mg/l
Details on test conditions:
- Test vessels: 5 liters, all-glass
- Test medium: ISO-medium, aerated until the dissolved oxygen concentration had reached saturation and the pH had stabilized.
- Number of fish: 7 fish exposed to 100 mg/L and teh controls. 3 fish exposed to 0.1, 1.0, and 10 mg/l.
- Loading: 0.4-0.6 g fish/liter, i.e. 3 fish per 2 litres or 7 fish per 3 litres of test medium.
- Illumination: 16 hours photoperiod daily
- Aeration: The test media were not aerated during the test
- Feeding: No feeding from 24 hours prior to the test and during the total test period.
- Introduction of fish: Directly after preparation of the test media.
- Euthanasia: At the end of the test the surviving fish were rapidly killed by exposing them to ca. 1.2% ethylene glycol monophenylether in water.
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
Not applicable
Sublethal observations / clinical signs:

solved oxygen values measured at the start and the end of the test. Temperature of the medium ranged from 20.5 to 20.6°C during the test.


Table1: Incidence and total mortality:










































































 Concentration (mg/L) Initial number of fish          Cumulative mortality Total mortality (%)
      24 h48 h 72 h 96 h   Total mortality (%)
 Blanl-control 7 0 0 0 0 0
 Solvent control 7 0 0 0 0 0
 0.1 3 0 0 0 0 0
 1.0 3 0 0 0 0 0
 10 3 1 1 1 1 33
 100 7 0* 0* 1* 1* 10

*Substance desposits were observed in this level.


 


Table 2: Incidence of clinical effects:










































































 Concentration (mg/L) Initial number of fish          Incidence of clinical effects Total affected (%)
   24 h 48 h 72 h 96 h   Total affected (%)
 Blank-control 7 0 0 0 0 0
 Solvent-control 7 0 0 0 0 0
 0.1 3 0 0 0 0 0
 1.0 3 0 0 0 0 0
 10 3 0 H=2/2 H=2/2H=2/2  100
 100 7 H=7/7 H=7/7 H=6/6 H=2/2 100

H: Number of fish that were hypoactive compared to the controls.


 


 


Table 3: pH and dissolved oxygen:






















































 Concentration mg/L Day 0 pH Day 0 DO Day 4 pH Day 4 DO
 Blank-control 8.1 9.1 7.9 8.4
 Solvent-control 8.1 9.1 - -
 0.1 8.1 9.1 - -
 1.0 8.1 9.1 - -
 10 8.1 9.1 - -
 100 8.1 9.1 7.4 8.4

DO: Dissolved oxygen


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 


 

Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the nominal 96h-LC50 was >100 mg/L.
Executive summary:

A study was conducted to determine the acute toxicity of the test substance, trichloro(N,N-dimethyloctylamine) boron, to Cyprinus carpio according to OECD Guideline 203 and EU Method C.1 without GLP. The definitive test was performed with carp exposed to concentrations ranging from 0.1 to 100 mg/L in a static system. Acetone was used as a solvent in the stock solution. The nominal 96 h LC50 was >100 mg/L. The maximum solubility was estimated to be between 10 and 100 mg/L. Hence, the testing of concentrations greater than 100 mg/L was not considered to be relevant. No analytical dose verification was conducted. Under the study conditions, the nominal 96 h LC50 was determined to be 100 mg/L (Bogers, 1996).

Description of key information

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
100 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance, trichloro(N,N-dimethyloctylamine) boron, to Cyprinus carpio according to OECD Guideline 203 and EU Method C.1 without GLP. The definitive test was performed with carp exposed to concentrations ranging from 0.1 to 100 mg/L in a static system. Acetone was used as a solvent in the stock solution. The nominal 96 h LC50 was >100 mg/L. The maximum solubility was estimated to be between 10 and 100 mg/L. Hence, the testing of concentrations greater than 100 mg/L was not considered to be relevant. No analytical dose verification was conducted. Under the study conditions, the nominal 96 h LC50 was determined to be 100 mg/L (Bogers, 1996).