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Three acute studies covering three trophic levels have been conducted (fish, algae and daphnia) for 5-ethyl-1,3-dioxane-5-methanol. The L(E)C/50 values are provided below:

Fish (zebra fish) = >1000 mg/L

Daphnia = 2466 mg/L

Algae (Pseudokirchneriella subcapitata) = >1000 mg/L

In the chemical safety assessment performed according to Article 14(3) in connection with Annex I section 3 (Environmental Hazard Assessment) no hazard was identified. Therefore according to REACH Annex I (5.0) exposure estimation is not necessary. Consequently, in accordance with Column 2 of REACH Annex IX, long term studies do not need to be conducted as all identified uses of the substance are assessed as safe for the environment.