Diethyl phosphonate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: similar to guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Male and female Wistar rats were shaved prior to dermal treatment with test substance in cellulose for 24 hours. The treated area was covered with bandage round back and belly. After removing of the bandage the treated area was washed with soap and water . the post exposure observation time was 14 days,animals were observed for skin effects, clinical signs and mortality. The LD50 was calculated according to Lichtfield and Wilcoxon, 1949

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl phosphonate

EC Number:

212-091-6

EC Name:

Diethyl phosphonate

Cas Number:

762-04-9

Molecular formula:

C4H11O3P

IUPAC Name:

diethyl phosphonate

Test material form:

other: colourless liquid

Details on test material:

content not given
the substance is described as technically pur colourless liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 160 - 200 g
- Fasting period before study: ca 16 hours
- Diet ad libitum:
- Water ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
no details given

Administration / exposure

Type of coverage:

occlusive

Vehicle:

other: cellulose

Details on dermal exposure:

Male and female Wistar rats were shaved prior to dermal treatment with test substance in cellulose for 24 hours. The treated area was covered with bandage round back and belly. After removing of the bandage the treated area was washed with soap and water . the post exposure observation time was 14 days,animals were observed for skin effects, clinical signs and mortality. The LD50 was calculated according to Lichtfield and Wilcoxon, 1949

Duration of exposure:

24 hours

Doses:

1000, 2500, 3500, 5000, 5500(females only), 6000, 6500 (males only) µl/kg bw

No. of animals per sex per dose:

5 males and 5 females , each group treated with 1000 and 2500 µl/kg bw, respectively
for all other doses: 10 males and 10 females

Control animals:

no

Details on study design:

Male and female Wistar rats were shaved prior to dermal treatment with test substance in cellulose for 24 hours. The treated area was covered with bandage round back and belly. After removing of the bandage the treated area was washed with soap and water . the post exposure observation time was 14 days,animals were observed for skin effects, clinical signs and mortality. The LD50 was calculated according to Lichtfield and Wilcoxon, 1949

Statistics:

The LD50 was calculated according to Lichtfield and Wilcoxon, 1949

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 4 695 other: µl/kg bw

95% CL:

>= 3 833 - <= 5 751

Remarks on result:

other: ca. 5071 mg/kg bw

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 4 850 other: µl/kg bw

95% CL:

>= 4 170 - <= 5 641

Remarks on result:

other: ca. 5238 mg/kg bw

Mortality:

no rat died following treatment with 1000 and 2500 µl/kg bw
male:
3500 µl/kgbw: 1/10 within 5 days; 5000 µl/kgbw: 5/10 within 1-3 days
6000 µl/kgbw: 9/10 within 1-2 days; 6500 µl/kgbw: 10/10 within 1 day
female
3500 µl/kgbw:2/10 within 2-4 days; 5000 µl/kgbw:4/10 within 2 days
5500 µl/kgbw:7/10 within1-2days; 6500 µl/kgbw: 10/10 within 1-2 days

Clinical signs:

other: males and females treated with 1000 µl/kg bw: showed no clinical signs all others:apathy, rough fur, prone position, sedation, reduced motility, accelerated breathing skin: the treated area was very strong irritated and necrotic , but during the post ex

Gross pathology:

animals that died during the observation period showed distended lungs, pale liver, kidneys and spleens with partly spotted surface

Applicant's summary and conclusion

Executive summary:

Male and female Wistar rats were shaved prior to dermal treatment with test substance in cellulose for 24 hours. The treated area was covered with bandage round back and belly. After removing of the bandage the treated area was washed with soap and water . the post exposure observation time was 14 days,animals were observed for skin effects, clinical signs and mortality. The LD50 was calculated according to Lichtfield and Wilcoxon, 1949. The LD50(dermal, male rat) was 4695 µl/kg bw (ca.5071 mg/kg bw) and LD50 (dermal, female) 4850 µl//kg bw ca. (5238 mg/kg bw)