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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information


In a 96-h acute toxicity study, rainbow trout (Oncorhynchus mykiss) were exposed to TEPA (Triethyl phosphono acetate) at 100 mg/L nominal concentrations under semi-static conditions according to the OECD guideline 203 (1992). 

The 96-h LC50 was >100 mg/L

The NOEL value, based on mortality/sublethal effects, was 100 mg/L.

No sublethal effects were observed. Based on the results of this study, TEPA would be not classified for fish. 

LC50 >100 mg /L      

NOEL = 100 mg /L


Daphnia magna:

The 48–hr-acute toxicity of TEPA to Daphnia magna was studied under static conditions according to OECD guideline 202.  Daphnids were exposed to control and test chemical at measured concentration of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hr.  Mortality was observed daily. 

The 48–hour EC50 was 3.9 mg/L. (3.6 -4.3 mg/L)

The study satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

EC50 3.9 mg /L

NOEL 1.8 mg /L


Algae :

In a 72 hour acute toxicity study, cultures of Pseudikirchneriella subcapitata were exposed to TEPA at nominal concentrations of 100 mg/L under static conditions in accordance with the OECD guideline 201.

The following results were determined:

NOEC = 100 mg/L

ErC50(0 -72h) > 100 mg/L

EyC50(0-72h) > 100 mg/L

In the both study (pre-test and main study), no inhibition on the growth of algae occured. This toxicity study is classified as acceptable and satisfies the guideline requirements for toxicity study.


Micro organism:

The inhibitory effect of the substance TEPA on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to OECD guideline 209 (2010).

The nominal concentrations tested was 1000 mg/L in a definitive study (limit test) after a range finding test at 10, 100 and 1000 mg/L (nominal concentration)

In addition, two controls and three different concentrations of the reference item 3,5-dichlorophenol (3.2, 10 and 32 mg/L) were tested in parallel. The results of these reference treatments (EC50 = 9.2 mg/L, falling within the guideline-recommended range of 5 - 30 mg/L, confidence limits 7.2 -12 mg/L) confirmed suitability of the activated sludge.

EC50 > 1000 mg/L

NOEC = 1000 mg/L

This study is classified as acceptable and satisfies the guideline requirements for toxicity study to activated sludge in a respiration inhibition test.