Registration Dossier
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EC number: 202-728-6 | CAS number: 99-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
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- pH
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- Additional physico-chemical information
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- Endpoint summary
- Stability
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Acute tox values:
oral
LD50 = 2121 mg/kg bw male
LD50 = 1784 mg/kg bw female
Inhalative: LD50= 880 mg/m³
Dermal: LD50 > 1157 mg/kg bw
Key value for chemical safety assessment
Additional information
With a single oral administration to rats, the following symptoms occurred at doses of 500 mg/kg bw and above: sedation, cyanosis, impairment of the general condition and patchy livers in animals found dead [Gröning et al. 1976.Bayer AG. Unpublished report]. Doses ranging from 1000 to 4000 mg/kg bw also caused excitation, increased breathing frequency, short-term convulsions, debility and atony [Ciss et al., 1980. Dakar Medical 25:303-311; Ciss et al., 1978. Dissertation. Unversite´ Rene´Descartes de Paris, Serie E No. 17].
In rats of both sexes a single dermal application of 1157 mg m-nitrotoluene/kg bw (exposure time: 24 hours) caused an impairment of general condition; no deaths occurred [Gröning et al. 1976.Bayer AG. Unpublished report]. A rabbit weighing 2.175 kg, treated with a single dermal application of 1 mL m-nitrotoluene (532 mg/kg bw) to the skin of the back, flanks and belly, exhibited a slightly increased breathing rate. The animal behaved normally during the 50-hour exposure period [Dambleff J: Beiträge zur Kenntnis der giftigen Wirkung nitrierter Benzole und Toluole insbesonder von der Haut aus. Dissertation, Würzburg, 1908].
A 1-hour inhalation of m-nitrotoluene vapour (calculated concentration: 2417 mg/m³) was tolerated without symptoms by male rats and mice [Gröning et al. 1976.Bayer AG.Unpublished report].
Study on Methemoglobin formation: Two cats received a single intra peritoneal injection of 100 mg m-nitroroluene/ kg bw, maximum methemoglobin levels ranging from 9 to 52% were reached after 4 to 8 hours; the values differed greatly between individual animals and no dose dependency was seen. More Heinz bodies were also detected that with o-nitrotoluene. All the treated animals displayed reduced blood-clotting ability. At doses of 300 mg/kg bw and above, the following effects were observed: cramps in the extremities, trembling, baring of the teeth, breathing disorders, dilation of pupils, rigidity of the entire musculature on the day of administration and muscular laxity on the following day [Von Bredow et al., Naunyn-Schmiedeberg´s Arch. Exp. Path. Pharmak. 200, 335-355. 1942-1943].
Justification for classification or non-classification
Like other nitrotoluenes, a primary toxic effect of 3 -nitrotoluene is methaemoglobin formation. Taking into account that humans are much more sensitive to methaemoglobin producing substances than rats it is suggested to classify 3-nitrotoluene as T; R 23/24/25 , according to DSD-DPD and Toxicity Category III according to EU-GHS.
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