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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Testing for the toxicity of chemicals with Tetrahymena pyriformis
Author:
Yoshioka Y, Ose Y, and Sato T
Year:
1985
Bibliographic source:
Sci. Total Environ. 43, 149-157

Materials and methods

Principles of method if other than guideline:
other: cell multiplication inhibition test
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
3-nitrotoluene
Details on test material:
- Name of test material (as cited in study report): m-Nitrotoluene
- Analytical purity: "analytical grade"

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
- Stock solution of test substance was added to the sterile medium to provide a concentration ratio
of 1.8 in 10 ml of 2 % proteose peptone.
- The solutions were then inoculated with 0.2 ml of the pre-cultured T. pyriformis and cultivated at
a temperature of 30 °C for 24 h without agitation.

Test organisms

Test organisms (species):
Tetrahymena pyriformis
Details on inoculum:
- Tetrahymena pyriformis was preserved in a sterile medium (of 2 % proteose peptone at a temperature of 20 °C) which was renewed at 2-4 week intervals.
- Tetrahymena pyriformis was pre-cultured at a temperature of 30 °C for 24 h.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
30 °C

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
50 mg/L
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion

Executive summary:

A cell-multiplication-inhibition test on the acute toxicity of 3-nitrotoluene to Tetrahymena pyriformis was performed for a test period of 24 hours. An EC50 value of 50 mg/l was reported (Yoshioka et al., 1985).