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Ecotoxicological information

Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms, other
Type of information:
other: BUA report
Adequacy of study:
other information
Reliability:
other: BUA report
Rationale for reliability incl. deficiencies:
other: No reliability is given as this is a summary entry for the BUA report.
Principles of method if other than guideline:
BUA report
GLP compliance:
not specified

BUA report (1989):


Among the measurements made in a study on the effect of aromatic nitrocompounds on the bioluminescence of Photobacterium phosphoreum was the mononitrotoluene concentration which causes a 50% reduction in the bacterium's glowing intensity, compared to that of the control, after exposure for 15 minutes. The EC50value for m-nitrotoluene was 3.95 mg/l (Deneer et al., 1988).

Validity criteria fulfilled:
not specified
Executive summary:

BUA report (1989):


Among the measurements made in a study on the effect of aromatic nitrocompounds on the bioluminescence of Photobacterium phosphoreum was the mononitrotoluene concentration which causes a 50% reduction in the bacterium's glowing intensity, compared to that of the control, after exposure for 15 minutes. The EC50value for m-nitrotoluene was 3.95 mg/l (Deneer et al., 1988).

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Principles of method if other than guideline:
other: cell multiplication inhibition test
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): m-Nitrotoluene
- Analytical purity: "analytical grade"
Analytical monitoring:
no
Details on test solutions:
- Stock solution of test substance was added to the sterile medium to provide a concentration ratio
of 1.8 in 10 ml of 2 % proteose peptone.
- The solutions were then inoculated with 0.2 ml of the pre-cultured T. pyriformis and cultivated at
a temperature of 30 °C for 24 h without agitation.
Test organisms (species):
Tetrahymena pyriformis
Details on inoculum:
- Tetrahymena pyriformis was preserved in a sterile medium (of 2 % proteose peptone at a temperature of 20 °C) which was renewed at 2-4 week intervals.
- Tetrahymena pyriformis was pre-cultured at a temperature of 30 °C for 24 h.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Test temperature:
30 °C
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
50 mg/L
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
not specified
Executive summary:

A cell-multiplication-inhibition test on the acute toxicity of 3-nitrotoluene to Tetrahymena pyriformis was performed for a test period of 24 hours. An EC50 value of 50 mg/l was reported (Yoshioka et al., 1985).

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Principles of method if other than guideline:
other: cell multiplication inhibition test according to Bringmann (1978)
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3-Nitrotoluol
- Analytical purity: no data
Analytical monitoring:
no
Test organisms (species):
Uronema parduzci
Total exposure duration:
20 h
Test temperature:
25 °C
pH:
6.9 (adjusted)
Duration:
20 h
Dose descriptor:
other: EC5
Effect conc.:
24 mg/L

Reported TT (Toxicity Threshold) is comparable to EC5; value refers to nominal TS concentration.

Validity criteria fulfilled:
not specified
Executive summary:

A cell multiplication inhibition test according to Bringmann (1978) on the acute toxicity of 3-nitrotoluene to Uronema parduzci was performed for a test period of 20 hours. An EC5 value of 24 mg/l was reported (Bringmann, 1980, 1981).

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Principles of method if other than guideline:
other: cell multiplication inhibition test according to Bringmann (1978)
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3-Nitrotoluol
- Analytical purity: no data
Analytical monitoring:
no
Test organisms (species):
Chilomonas paramaecium
Total exposure duration:
48 h
Test temperature:
25 °C
pH:
6.9 (adjusted)
Duration:
48 h
Dose descriptor:
other: EC5
Effect conc.:
48 mg/L

Reported TT (Toxicity Threshold) is comparable to EC5; value refers to nominal TS concentration.

Validity criteria fulfilled:
not specified
Executive summary:

A cell multiplication inhibition test according to Bringmann (1978) on the acute toxicity of 3-nitrotoluene to Chilomonas paramaecium was performed for a test period of 48 hours. An EC5 value of 48 mg/l was reported (Bringmann, 1980).

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Principles of method if other than guideline:
other: cell multiplication inhibition test according to Bringmann (1978)
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3-Nitrotoluol
- Analytical purity: no data
Analytical monitoring:
no
Test organisms (species):
Entosiphon sulcatum
Details on inoculum:
- Stock and preliminary cultures of protozoa are fed with viable bacteria (Escherichia coli).
Total exposure duration:
72 h
Test temperature:
25 °C
pH:
6.9 (adjusted)
Details on test conditions:
- The number of protozoa is determined by means of a cell counter (Coulter counter) after addition of
a suitable electrolyte (10 % of a 1.1 % NaNO3 solution in double-distilled water filtered through a
membrane filter (pore size = 0.2 µm)
Duration:
72 h
Dose descriptor:
other: EC5
Effect conc.:
12 mg/L

Reported TT (Toxicity Threshold) is comparable to EC5; value refers to nominal TS concentration.

Validity criteria fulfilled:
not specified
Executive summary:

A cell multiplication inhibition test according to Bringmann (1978) on the acute toxicity of 3-nitrotoluene to Entosiphon sulcatum was performed for a test period of 72 hours. An EC5 value of 12 mg/l was reported (Bringmann, 1980, 1981).

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Principles of method if other than guideline:
cell multiplication inhibition test according to Bringmann and Kuehn
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3-Nitrotoluol
- Analytical purity: no data
Analytical monitoring:
no
Test organisms (species):
Pseudomonas putida
Total exposure duration:
16 h
Test temperature:
25 °C
pH:
7
Duration:
16 h
Dose descriptor:
other: EC3
Effect conc.:
10 mg/L

Reported TT (Toxicity threshold) is comparable to EC3; value refers to nominal TS concentration.

Validity criteria fulfilled:
not specified
Executive summary:

A cell multiplication inhibition test according to Bringmann (1976, 1977, 1979, 1980) on the acute toxicity of 3-nitrotoluene to Pseudomonas putida was performed for a test period of 16 hours. An EC3 value of 10 mg/l was reported (Bringmann, 1976, 1977, 1979, 1980).

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Unsuitable test system; important information missing (e.g. quality criteria)
Principles of method if other than guideline:
Microtox test applied according to manual of analyzer (Model Toxicity Analyzer DXY-2 of the Institute of Soil Science, Academia Sinica, Nanjing)
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3-Nitrotoluene
- Analytical purity: > 95%
Analytical monitoring:
no
Test organisms (species):
Photobacterium phosphoreum
Water media type:
freshwater
Total exposure duration:
15 min
Test temperature:
20°C
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
24.95 mg/L

- Results are given in mol/l
- It is reported that also tests with an incubation period of 30 min had been performed, and that the results were similar to these performed with a 15 min incubation period
- Unfortunately method only partly described

Validity criteria fulfilled:
not specified
Executive summary:

A microtox test was applied according to manual of the analyzer (Model Toxicity Analyzer DXY-2 of the Institute of Soil Science, Academia Sinica, Nanjing) in order to investigate the acute toxicity of 3-nitrotoluene to Phosphobacterium phosphoreum. An EC50 (15 min) value of 24.95 mg/l was reported (Zhao, 1995).

Description of key information

For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".


A cell-multiplication-inhibition test on the acute toxicity of 3-nitrotoluene to Tetrahymena pyriformis was performed for a test period of 24 hours. An EC50 value of 50 mg/l was reported (Yoshioka et al., 1985).


BUA report (1989): Among the measurements made in a study on the effect of aromatic nitrocompounds on the bioluminescence of Photobacterium phosphoreum was the mononitrotoluene concentration which causes a 50% reduction in the bacterium's glowing intensity, compared to that of the control, after exposure for 15 minutes. The EC50value for m-nitrotoluene was 3.95 mg/l (Deneer et al., 1988).


A cell multiplication inhibition test according to Bringmann (1978) on the acute toxicity of 3-nitrotoluene to Uronema parduzci was performed for a test period of 20 hours. An EC5 value of 24 mg/l was reported (Bringmann, 1980, 1981).


A cell multiplication inhibition test according to Bringmann (1978) on the acute toxicity of 3-nitrotoluene to Entosiphon sulcatum was performed for a test period of 72 hours. An EC5 value of 12 mg/l was reported (Bringmann, 1980, 1981).


A cell multiplication inhibition test according to Bringmann (1978) on the acute toxicity of 3-nitrotoluene to Chilomonas paramaecium was performed for a test period of 48 hours. An EC5 value of 48 mg/l was reported (Bringmann, 1980).


A cell multiplication inhibition test according to Bringmann (1976, 1977, 1979, 1980) on the acute toxicity of 3-nitrotoluene to Pseudomonas putida was performed for a test period of 16 hours. An EC3 value of 10 mg/l was reported (Bringmann, 1976, 1977, 1979, 1980).


A microtox test was applied according to manual of the analyzer (Model Toxicity Analyzer DXY-2 of the Institute of Soil Science, Academia Sinica, Nanjing) in order to investigate the acute toxicity of 3-nitrotoluene to Phosphobacterium phosphoreum. An EC50 (15 min) value of 24.95 mg/l was reported (Zhao, 1995).

Key value for chemical safety assessment

Additional information