3-nitrotoluene

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given (comparable to guidelines/standards)

Data source

Reference

Reference Type:

publication

Title:

Toxicity, biodegrability and accumulation of a number of Cl/N-containing compounds for classification and establishing Water Quality Criteria

Author:

Canton JH, Slooff W, Kool HJ, Struys J, Gouw TJM, Wegman RCC, Piet GJ.

Year:

1985

Bibliographic source:

Regul. Toxicol. Pharmacol. 5: 123-131

Materials and methods

Principles of method if other than guideline:

in analogy with the OECD 202 proposal 1979.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-nitrotoluene

EC Number:

202-728-6

EC Name:

3-nitrotoluene

Cas Number:

99-08-1

Molecular formula:

C7H7NO2

IUPAC Name:

1-methyl-3-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: 99.5 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

- Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnias were 1 day old at start of incubation
- Food during incubation: Chlorella
- 25 organisms per 1 litre of test medium, 2 replicates

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

- Incubation temperature 19 ± 1 °C

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

3.5 mg/L

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.5 mg/L

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

9 mg/L

Basis for effect:

other: mortality + behaviour

Applicant's summary and conclusion

Executive summary:

The toxicity of 3 -Nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.