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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given (comparable to guidelines/standards)

Data source

Reference
Reference Type:
publication
Title:
Toxicity, biodegrability and accumulation of a number of Cl/N-containing compounds for classification and establishing Water Quality Criteria
Author:
Canton JH, Slooff W, Kool HJ, Struys J, Gouw TJM, Wegman RCC, Piet GJ.
Year:
1985
Bibliographic source:
Regul. Toxicol. Pharmacol. 5: 123-131

Materials and methods

Principles of method if other than guideline:
in analogy with the OECD 202 proposal 1979.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
1-methyl-3-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: 99.5 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
- Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnias were 1 day old at start of incubation
- Food during incubation: Chlorella
- 25 organisms per 1 litre of test medium, 2 replicates

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
- Incubation temperature 19 ± 1 °C

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
3.5 mg/L
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.5 mg/L
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
9 mg/L
Basis for effect:
other: mortality + behaviour

Applicant's summary and conclusion

Executive summary:

The toxicity of 3 -Nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.