Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-728-6 | CAS number: 99-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given (comparable to guidelines/standards)
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicity, biodegrability and accumulation of a number of Cl/N-containing compounds for classification and establishing Water Quality Criteria
- Author:
- Canton JH, Slooff W, Kool HJ, Struys J, Gouw TJM, Wegman RCC, Piet GJ.
- Year:
- 1 985
- Bibliographic source:
- Regul. Toxicol. Pharmacol. 5: 123-131
Materials and methods
- Principles of method if other than guideline:
- in analogy with the OECD 202 proposal 1979.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-nitrotoluene
- EC Number:
- 202-728-6
- EC Name:
- 3-nitrotoluene
- Cas Number:
- 99-08-1
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- 1-methyl-3-nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: 99.5 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- - Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Daphnias were 1 day old at start of incubation
- Food during incubation: Chlorella
- 25 organisms per 1 litre of test medium, 2 replicates
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- - Incubation temperature 19 ± 1 °C
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 3.5 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.5 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 9 mg/L
- Basis for effect:
- other: mortality + behaviour
Applicant's summary and conclusion
- Executive summary:
The toxicity of 3 -Nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live1