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Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Toxicity, biodegrability and accumulation of a number of Cl/N-containing compounds for classification and establishing Water Quality Criteria
Author:
Canton JH, Slooff W, Kool HJ, Struys J, Gouw TJM, Wegman RCC, Piet GJ.
Year:
1985
Bibliographic source:
Regul. Toxicol. Pharmacol. 5: 123-131

Materials and methods

Principles of method if other than guideline:
according to Canton and Sloof (1982)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitrotoluene
EC Number:
202-728-6
EC Name:
3-nitrotoluene
Cas Number:
99-08-1
Molecular formula:
C7H7NO2
IUPAC Name:
3-nitrotoluene
Details on test material:
- Name of test material (as cited in study report): 3-Nitrotoluene
- Analytical purity: > 99.5 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
- Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
- 50 animals (3 - 4 weeks old) each test
- Fish were fed with Tetramine, Tetraphyll

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
28 d

Test conditions

Test temperature:
23 ± 2 °C
Details on test conditions:
- 3-times a week adjustment of the concentration

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
2 mg/L
Conc. based on:
test mat.
Basis for effect:
other: mortality + behaviour
Duration:
28 d
Dose descriptor:
LC50
Effect conc.:
9.9 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
additional parameter: 28d EC50 (mortality + behaviour): 3.0 mg/l

Applicant's summary and conclusion

Executive summary:

The toxicity of 3 -Nitrotoluene to Oryzias latipes was evaluated in a 28 d study performed according to the methodology described by Canton and Sloof (1982). The 28d-NOEC value was determined to be 2 mg/L (Canton et al. 1985).