3-nitrotoluene

Long-term toxicity to fish

Administrative data

Endpoint:

long-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Toxicity, biodegrability and accumulation of a number of Cl/N-containing compounds for classification and establishing Water Quality Criteria

Author:

Canton JH, Slooff W, Kool HJ, Struys J, Gouw TJM, Wegman RCC, Piet GJ.

Year:

1985

Bibliographic source:

Regul. Toxicol. Pharmacol. 5: 123-131

Materials and methods

Principles of method if other than guideline:

according to Canton and Sloof (1982)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-nitrotoluene

EC Number:

202-728-6

EC Name:

3-nitrotoluene

Cas Number:

99-08-1

Molecular formula:

C7H7NO2

IUPAC Name:

1-methyl-3-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 3-Nitrotoluene
- Analytical purity: > 99.5 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

- Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

- 50 animals (3 - 4 weeks old) each test
- Fish were fed with Tetramine, Tetraphyll

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Test conditions

Test temperature:

23 ± 2 °C

Details on test conditions:

- 3-times a week adjustment of the concentration

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

2 mg/L

Conc. based on:

test mat.

Basis for effect:

other: mortality + behaviour

Effect concentrations 2

Duration:

28 d

Dose descriptor:

LC50

Effect conc.:

9.9 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

additional parameter: 28d EC50 (mortality + behaviour): 3.0 mg/l

Applicant's summary and conclusion

Executive summary:

The toxicity of 3 -Nitrotoluene to Oryzias latipes was evaluated in a 28 d study performed according to the methodology described by Canton and Sloof (1982). The 28d-NOEC value was determined to be 2 mg/L (Canton et al. 1985).