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EC number: 202-728-6 | CAS number: 99-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Biodegradation and bioaccumulation data of existing chemicals based on the Chemical Substances Control Law (CSCL)
- Author:
- MITI (Ministry of International Trade and Industry)
- Year:
- 1 992
- Bibliographic source:
- Chemicals Inspection and Testing Institute (CITI, ed.); Japan Chemicals Industry Ecology Toxicology and Information Center
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- 14 day-test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-nitrotoluene
- EC Number:
- 202-728-6
- EC Name:
- 3-nitrotoluene
- Cas Number:
- 99-08-1
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- 1-methyl-3-nitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): m-Nitrotoluene
- Analytical purity: no data
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: sludge sampling from different sewage plants, natural waterbodies and soil
- Details on inoculum:
- 1. Sludge sampling sites and time
1.1. Time: in March, June, September, and December, sludge was sampled
1.2. Sites (Japan): 1. Fukogawa city sewage plant, 2. Fukashiba industry sewage plant, 3. Nakahama city sewage plant, 4. Ochiai city sewage plant, 5. Kitakami river, 6. Shinano river, 7. Yoshino river, 8. Lake Biwa, 9. Hiroshima bay, 10. Dookai bay
A mixture composed of city and industry sewage and river, lake and sea surface water and soil was tested.
2. Sludge sampling method
2.1. City sewage: Returned sludge from sewage plants was taken.
2.2. Rivers, lake and sea: Surface water and surface soil which were in contact with atmosphere were collected.
3. Method of cultivation
3.1. Mixing of fresh and old activated sludge: 5 L of the filtrate of the supernatant of old activated sludge was mixed with 500 mL of the filtrate of the supernatant of new sludge and cultured at pH 7.0 ± 1.0 under sufficient aeration using prefiltered open air.
3.2. Culture: About 30 minutes after ceasing aeration to the sludge mixture, supernatant corresponding to about 1/3 of the whole volume was removed. Then the equal volume of dechlorinated water was added to the remaining portion and aerated again, followed by addition of synthetic sewage at aconcentration of 0.1% (w/v). This procedure was repeated once every day. The culturing was carried out at 25 ± 2 °C.
3.3. Control: During the cultivation, appearance of the supernatant, precipitability, formation of flock, pH, dissolved oxygen concentration in the solution and temperature were checked and necessary adjustments were made, Microflora in the activated sludge was microscopically observed and sludge with no abnormal symptom was used for the test.
4. Inspection of activity
Activity of the sludge was inspected to use reference substance. And the relation between new and old activated sludge was taken account.
5. Adaptation
There is no specific statement about the adaptation of the used microorganisms but the sampling sides and culture conditions suggest not adapted microorganisms.
6. Concentration of sludge: 30 mg/L - Duration of test (contact time):
- 14 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test performed in open system: no
Preparation of basal culture medium (sludge):
- Suspended solids concentration: determined according to Method Japanese Industrial Standards (JIS) K 0102-1986-14.1
- Composition of basal culture medium: 3 mL each of four stock solutions, as described in JIS K 0102-1986-21, are diluted to 1000 mL with purified water
- pH of basal culture medium: 7.0
- pH adjusted: yes
Test system:
- Abiotic sterile control: 300 ml purified water + 30 mg test substance
- Test solution: 300 ml basal culture medium (sludge) + 30 mg test substance
- Reference: 300 ml basal culture medium (sludge) + 30 mg aniline
- Inoculum blank: 300 ml sludge
Test instruments and conditions:
- Culturing apparatus: Closed system oxygen consumption measuring apparatus (Coulometer: Ohkura Electric Co., Ltd.)
- Vessel size: 300 mL in volume
- absorbent for evolving carbon dioxide: Soda lime No .l (extra pure reagent, Wako Pure Chemical Industries, Ltd.).
- stirring method: test solution was stirred by a magnetic stirrer
- cultivating temperature: 25 ± 1°C
- reference substance: aniline
Analysis after termination of the test:
- TOC
- test substance
- pH
Degradation (%) was obtained from the following equations:
BOD: Degradation (%) = (BOD-B)/ThOD x 100 BOD (mg):
BOD in (sludge + m-Nitrotoluene system)
B (mg): BOD in sludge blank
ThOD: theoretical oxygen demand
required when m-Nitrotoluene was completely oxidized.
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2
- Sampling time:
- 14 d
Applicant's summary and conclusion
- Interpretation of results:
- other: not readily biodegradable
- Executive summary:
In a modified MITI I test according to OECD guideline 301 C a non adapted mixed microbial inoculum mineralised 2 % of the initial test substance concentration within 14 days (MITI, 1992).
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