Tris[4-(diethylamino)phenyl]methylium acetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Principles of method if other than guideline:

Method: BASF-Test. In principle, the methods described in OECD Guideline 401 were used.
5 rats per sex and dose were treated simultaneously by gavage with preparations of the test substance in water. Group-wise documentation of clinical signs was performed over the 7- to 14-day study period. The clinical signs and findings were reported in summary form.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tris[4-(diethylamino)phenyl]methylium acetate

EC Number:

263-974-8

EC Name:

Tris[4-(diethylamino)phenyl]methylium acetate

Cas Number:

63157-72-2

Molecular formula:

C31H42N3.C2H3O2

IUPAC Name:

tris[4-(diethylamino)phenyl]methylium acetate

Details on test material:

- Name of test material (as cited in study report): Ethylviolett fluessig

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

316, 464, 681, 1000 mg/kg bw, corresponding to ca. 126, 186, 272, 400 mg/kg bw EVA.

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Other examinations performed: clinical signs, body weights

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 410 mg/kg bw

Based on:

other: preparation containing ca. 40% ethylviolet acetate, ca. 17% acetic acid, ca. 17% confidential component and ca. 26% water

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 234 mg/kg bw

Based on:

other: registered substance

Clinical signs:

other: Poor general state with staggering in some animals, atony, paresis, narcotic-like state with absence of pain reflex, tremors, spastic gait, diarrhea and salivation and occasionally substantial loss of weight at the beginning.

Gross pathology:

NECROPSY FINDINGS:
Animals that died:
- heart: acute dilatation
- acute passive hyperemia
- stomach: dilated and liquid contents
- organs/muscles/adipose tissue: colored
- intestines: liquid contents in some animals and atonic
Sacrificed animals:
- organs: no abnormalities detected

Any other information on results incl. tables

MORTALITY DATA:

Male animals:

Dose (test material) (mg/kg)	Dose (act. ingr.) (mg/kg)	Dead animals/animals per group after
		1 h	24 h	48 h	7 d	14 d
316	126	0/5	0/5	0/5	0/5	0/5
464	186	0/5	1/5	2/5	3/5	3/5
681	272	0/5	0/5	1/5	4/5	4/5
1000	400	0/5	1/5	5/5	5/5	5/5

  

Female animals:

Dose (test material) (mg/kg)	Dose (act. ingr.) (mg/kg)	Dead animals/animals per group after
		1 h	24 h	48 h	7 d	14 d
316	126	0/5	1/5	1/5	2/5	2/5
464	186	0/5	1/5	4/5	4/5	4/5
681	272	0/5	2/5	3/5	4/5	5/5
1000	400	0/5	4/5	5/5	5/5	5/5