Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
DOSE doc no : D399
Author:
RSC Publishing
Year:
2011
Bibliographic source:
Dictionary of Substances and their effects (DOSE):-Kuehn, R. Water Res. 1990, 24(1), 31-38.
Reference Type:
publication
Title:
Results of the Harmful Effects of Water Pollutants to Green Algae (Scenedesmus subspicatus) in the Cell Multiplication Inhibition Test
Author:
Kuhn, R., and M. Pattard
Year:
1990
Bibliographic source:
Water Res.24(1): 31-38

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other:
Principles of method if other than guideline:
In the Scenedesmus cell multiplication inhibition test, 68 potentially hazardous substances were examined to determine the effect concentrations (EC). The tests were conducted in accordance with the test procedure DIN 38 412, Part 9 (draft standard). The green alga Scenedesmus subspicatus CHODAT was cultivated as the test organism. Twenty-five substances were examined according to the standardized test procedure. The procedure was modified somewhat for the examination of volatile and/or strongly smelling substances.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
24 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
not specified

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 values to algae, mg/l : 24 ,48hr, biomass, Scenedesmus subspicatus.
Toxicity to aquatic algae (Scenedesmus subspicatus) (48h) examined at dose concentration 24 mg/L.
Executive summary:
In the Scenedesmus cell multiplication inhibition test, 68 potentially hazardous substances were examined to determine the effect concentrations (EC). The tests were conducted in accordance with the test procedure DIN 38 412, Part 9 (draft standard). The green alga Scenedesmus subspicatus CHODAT was cultivated as the test organism. Twenty-five substances were examined according to the standardized test procedure. The procedure was modified somewhat for the examination of volatile and/or strongly smelling substances. For 21 of the tested substances the concentrations for the 72 h and/or 48 h EBC10 values were in the concentration range 0.0001-2 mg1-1. Thus, they proved very/highly toxic to the cell multiplication of Scenedesmus subspicatus. When compared with the results of the 21d Daphina reproduction test, monobromoacetic, monochloroacetic acid, chloroacetaldehyde and chloramine T proved very harmful to green algae.