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EC number: 939-651-9 | CAS number: 1474044-70-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Protocols and results reviewed and accepted by the National Toxicology Program's Board of Scientific Counselor's Technical Reports Review Subcommittee, USA National Institutes of Health
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 870.5300 - In vitro Mammalian Cell Gene Mutation Test
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- sodium xylenesulfonate
- IUPAC Name:
- sodium xylenesulfonate
- Reference substance name:
- Sodium xylenesulphonate
- EC Number:
- 215-090-9
- EC Name:
- Sodium xylenesulphonate
- Cas Number:
- 1300-72-7
- Molecular formula:
- C8H10O3S.Na
- IUPAC Name:
- sodium dimethylbenzenesulfonate
- Details on test material:
- - Name of test material (as cited in study report): sodium xylenesulfonate
- Molecular formula (if other than submission substance): no data
- Molecular weight (if other than submission substance): no data
- Substance type: organic
- Physical state: powder
- Analytical purity: 65%
- Impurities (identity and concentrations): not identified from NMR spectrum provided
- Composition of test material, percentage of components: 11.5% ortho, 38% meta, 15.5% para
- Purity test date: February 13, 1986
- Lot/batch No.: RO92085 / 03
- Expiration date of the lot/batch: no data
- Stability under test conditions: stable
- Storage condition of test material: glass bottles with Teflon lined caps or double bagged in metal drums at room temperature in the dark
- Other:
Constituent 1
Constituent 2
Method
- Target gene:
- mouse lymphoma cells - TK locus
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- 125, 250, 500, 1000 and 2500 microgram active ingredient per milliliter
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: no data
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- Migrated to IUCLID6: without S9 and methlcholanthrene with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in Fischer medium
DURATION
Normal cycling time for L5178Y cells was 10 hours. Treated cultures contained 6 x 10-6 cells in 10mL medium.
Incubation with test substance continued for 4 hours, at which time the medium plus test substance was removed and the cells were resuspended in fresh medium and incubated for an additional 2 days to express the mutant phenotype. Cell density was maintained at log phase growth. After the 48-hr expression period, cells were plated in medium and soft agar supplemented with TFT (trifluorothymidine) for selection of TFT-resistant cells. Cells were plated in nonselective medium and soft agar to determine cloning efficiency. Plates were incubated at 37 degrees C in 5% CO2 for 10-12 days.
NUMBER OF REPLICATIONS: each treatment level was replicated, including positive and solvent controls
OTHER: - Evaluation criteria:
- Minimum criteria for accepting an experiment as valid are presented in Caspary et al 1988
- Statistics:
- All data were evaluated statistically for trend and peak responses. Both responses had to be significant (P<=0.05) for the test substance to be considered positive; i.e., capable of inducing TFT resistance. A single significant response led to a "questionable" conclusion, and absence of both a trend and a peak response resulted in a "negative" conclusion.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Negative in one replicate series and positive in other replicate series with metabolic activation; leads to "equivocal" conclusion with metabolic activation
- Remarks on result:
- other: strain/cell type: L5178Y
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation questionable with metabolic activation
Not mutagenic without metabolic activation, equivocal with metabolic activation
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