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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Inhibition of "spontaneous" mutagenicity in Salmonella typhimurium TA 102 and TA 104
Author:
De Flora S, Bennicelli C, Rovida A, Scatolini L, Camoirano A
Year:
1994
Bibliographic source:
Mutation Research, 307, 157-167

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Vanillin
EC Number:
204-465-2
EC Name:
Vanillin
Cas Number:
121-33-5
Molecular formula:
C8H8O3
IUPAC Name:
1-butoxypropan-2-ol

Method

Species / strain
Species / strain / cell type:
other: S. typhimurium :TA102 and TA104
Additional strain / cell type characteristics:
not specified
Metabolic activation:
without
Metabolic activation system:
no data
Test concentrations with justification for top dose:
2, 4, 6, 8 and 10 µmol/plate
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: no data
- Vehicle controls tested: no data
- volume of vehicle/solvent in the medium: 100 µL
Details on test system and experimental conditions:
- Positive control(s): no data
- Method of application: in agar (plate incorporation)
- Duration:
* Preincubation period: none
* Exposure duration : 48 hrs (at 37 °C)
- Number of replicates: triplicate plates, in at least 3 separate experiments
Statistics:
The anti-mutagenic activity was assessed by the Student's t-test

Results and discussion

Test results
Species / strain:
other: S. typhimurium : TA102 and TA104
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative without metabolic activation

The test subsatance vanillin is found to be negative on S. typhimurium : TA102 and TA104 strains without metabolic activation.
Executive summary:

The test subsatance vanillin is found to be negative on S. typhimurium : TA102 and TA104 strains without metabolic activation.