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Short-term toxicity to aquatic invertebrates

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Description of key information

Acutely very toxic for aquatic invertebrates

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.015 mg/L

Additional information

Tridecylamine, branched and linear (CAS 86089-17-0) is a mixture of C13 isomers (branched and linear; 99.8 -100%).

1 -Tridecanamine (CAS 2869 -34-3 = linear isomer) was selected as a representative C13 substance due t its expected higher toxicity (see Summary of IUCLID Ch. 6.1). Therefore, the assessment of the toxicity of the substance to aquatic invertebrates was based on the available experimental data of the compound itself and on the read-across data for the source chemical CAS 2869-34-3.

Effects of 1-tridecanamine (CAS 2869-34-3) on the mobility of daphnids were investigated in a 48 -h static study on Daphnia magna under GLP conditions and following OECD TG 202. Due to the low water solubility, the test concentrations were prepared as water accomodated fractions (WAF) as described in OECD TG 23. The analysis of test concentrations was conducted by GC-MS; the results are expressed as mean measured concentrations. The 48 -h EC50 based on measured test item concentrations on was 0.015 mg/L (Petersen et al., 2010). The study was considered as key study as it is a GLP guideline study and resulted in the lowest effect value .


Data are also available for TDA, b+l. The effects on daphnids were determined in two elder studies performed similarly to nowadays methods (according to DIN 38412, L11), but without analytical monitoring. Based on nominal concentrations, the 48 -h EC50 values on Daphnia magna were extrapolated by linear regression analysis to be 0.7 mg/L (BASF AG, 1989) and 0.08 mg/L (Amann, 1989). These studies were considered as supporting studies.


In addition, a further information on the toxicity to Daphnia magna are available from a non-GLP screening study with Daphnia magna which was conducted to study the potential mitigating effect of humic acid on the toxicity of TDA, b+l (BASF SE, 2016; report no. 85E0391/03E010). The screening study followed a modified protocol of OECD 202 (reduced number of test concentrations, higher spacing factor, no concentration control analysis). Test solutions were prepared as water accomodated fractions at loading rates of 1, 10 and 100 mg test item/L (nominal). The screening test was performed to evaluate the potential of natural carbon for toxicity mitigation. Humic acid was used to simulate the carbon source. One experiment was performed without addition of humic acid, while two others were performed with addition of humic acid (Na salt) at concentrations of 3 and 10 mg/L. The EL50 in the experiment without humic acid addition was estimated to be 1.20 mg/L, while in the experiment with addition of 3 mg/L humic acid, the EL50 was 1.50 mg/L and in the experiment with 10 mg/L the EL50 was 1.32 mg/L. It can be concluded that there was no clear trend in the estimated EL50 values; thus, the results demonstrate no potential mitigation through humic acid on the acute toxicity of tridecylamine, branched and linear. However, due to considerable restrictions (e.g. no analytical verification of test item concentrations, the high spacing factor, low number of replicates, low number of test item concentrations), the screening study is disregarded in terms of the chemical safety assessment.