3-chlorophenyl isocyanate

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

repeated dose toxicity: inhalation

Remarks:

other: 5 days repeated dose inhalation toxicity in 1 rabbit, 1 guinea pig, 2 rats and 4 mice

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: No OECD Guideline or GLP defined. Insufficient data documentation, insufficient number of test animals used.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1960

Report date:

1960

Materials and methods

Principles of method if other than guideline:

4 ccm of a 20% dilution of the test substance in Methylenchloride were sprayed in a 400 l room, in which 1 rabbit, 1 guinea pig , 2 rats and 4 mice were tested. After 1 hour the same amount of test substance was sprayed again. After 2 hours the animals were taken out of the testing room. This test was repeated on 5 consecutive days.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chlorophenyl isocyanate

EC Number:

220-822-5

EC Name:

3-chlorophenyl isocyanate

Cas Number:

2909-38-8

Molecular formula:

C7H4ClNO

IUPAC Name:

1-chloro-3-isocyanatobenzene

Details on test material:

no data

Test animals

Species:

other: rabbit, guinea pig, rats, mice

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

other: Methylenchloride

Remarks on MMAD:

MMAD / GSD: no data

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

5 days

Frequency of treatment:

twice daily (4 ccm of a 20% dilution of the test substance in Methylenchloride were sprayed in a 400 l room, in which 1 rabbit, 1 guinea pig , 2 rats and 4 mice were tested. After 1 hour the same amount of test substance was sprayed again. After 2 hours the animals were taken out of the testing room. This test was repeated on 5 consecutive days).

Doses / concentrations

Remarks:

Doses / Concentrations:
4 ccm
Basis:
no data

No. of animals per sex per dose:

1 rabbit, 1 guinea pig , 2 rats and 4 mice

Control animals:

not specified

Results and discussion

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

The animals reacted after the first spraying of test substance, a heavy mucosa irritation was observed together with difficulties in breathing. These syptoms intensified on day 2. On day 3 the guinea pig died, on day 4 one mouse died, an day 5 another mouse and one rat and 2 days after the end of the experiment the 2 other mice died. The rabbit died 7 days after the experiment and the 2 rats after 14 days.

Applicant's summary and conclusion

Executive summary:

In a repeated dose inhalation study 4 ccm of a 20% dilution of the test substance in Methylenchloride were sprayed in a 400 l room, in which 1 rabbit, 1 guinea pig , 2 rats and 4 mice were tested. After 1 hour the same amount of test substance was sprayed again. After 2 hours the animals were taken out of the testing room. This test was repeated on 5 consecutive days.

The animals reacted after the first spraying of test substance, a heavy mucosa irritation was observed together with difficulties in breathing. These syptoms intensified on day 2. On day 3 the guinea pig died, on day 4 one mouse died, an day 5 another mouse and one rat and 2 days after the end of the experiment the 2 other mice died. The rabbit died 7 days after the experiment and the 2 rats after 14 days.