Reaction mass of chlorodifluoroacetic acid and trifluoroacetic acid

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

from 14 dec 1989 to 18 apr 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study, OECD 402 compliant

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Chlorodifluoroacetic acid

EC Number:

200-928-8

EC Name:

Chlorodifluoroacetic acid

Cas Number:

76-04-0

Molecular formula:

C2HClF2O2

IUPAC Name:

chloro(difluoro)acetic acid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Iffa-Crédo (69592 L'Arbresle, France)
- Age at study initiation: 6 to 8 weeks old
- Weight at study initiation: 200 to 242 g
- Housing: individual in polycarbonte cages, typeFI (305x180x184 mm)
- Diet (e.g. ad libitum): ad libitum, rat-mice pellets (U.A.R, formule A.04 - U.A.R Villemoison, 91360 Epinay/Orge, France)
- Water (e.g. ad libitum): softened and filtered water (0.6µm), ad libitum
- Acclimation period: 6 days before

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 30 to 70%
- Air changes (per hr): 10 minimum
- Photoperiod (hrs dark / hrs light): 12h/12h

IN-LIFE DATES: from 02 jan 1990 to 09 jan 1990

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: back or flanks
- % coverage: 10%
- Type of wrap if used: semiocclusive gauze dressing under an elastic bandage (Creplux / Moliner)

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no
- Time after start of exposure: 24h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit):
1.31 ml/mg

Duration of exposure:

24 hours

Doses:

2004 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: 1/4 hour after application, then 1, 2 and 4 hours and daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, necropsy

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Based on:

test mat.

Remarks on result:

other: No LD 50 could be determined because product is corrosive

Mortality:

Animals were sacrified on day 2, for human reason because Chlorodifluoroacetic acid was corrosive.

Clinical signs:

other: None

Other findings:

Total necrosis of tissues including skin and muscles.

Applicant's summary and conclusion

Interpretation of results:

other: LD50 not determined becaude of corrosivity

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

The LD50 of Chlorodifluoroacetic acid could not be determined because of its corrosivity.

Executive summary:

In a GLP study, Chlorodifluoroacetic acid was tested to evaluate the acute toxicity following a single cutaneous adminitration at doses 0 and 2004 mg/kg bw undiluted.

Total necrosis of tissues including skin and muscles was observed at the treatment site.

In these conditions, the LD50 of Chlorodifluoroacetic acid could not be determined because of its corrosivity.