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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Based on experimental data on each constituent, the reaction mass is considered as harmful to fish with a 96h LC50 between 60 and 100 mg/L. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
60 mg/L

Additional information

No data on acute toxicity to fish is available on the reaction mass of chlorodifluoroacetic acid (CDFA) and trifluoroacetic acid (TFA).

The following data are available on each constituent:

For CDFA, in the preliminary range-finding study of a 14-day fish toxicity test, groups of 10 fish (Danio rerio) were exposed to the test solutions with nominal active ingredient concentration of 100, 80 and 60 mg/L. Synchronously one control group was conducted in test water without the test item. Test duration was 96 hours. Once the test started, the mortalities of the fish were recorded at 24, 48, 72 and 96h. Dead fish were removed at least once daily and discarded. Mortality was observed as follows: 30% at 60 mg/L, 50% at 80 mg/L and 100% at 100 mg/L. Based on these results, the LC50 (96h) was considered between 60 and 100 mg/L.

These results were confirmed in the main study which shows, during 14 days of exposure, no mortality at 40 mg/l and 30% mortality at the highest tested concentration of 60 mg/L (see the section on fish long term toxicity data). Therefore CDFA is harmful to fish.

For TFA, in a 96-h acute toxicity study under static conditions following the OECD Guideline 203, Zebrafish (Danio rerio, n=10) were exposed in a limit test at 1200 and 0 mg/l of trifluoroacetate sodium (i.e. 999 mg/l of TFA) in duplicates. No mortality was observed, demonstrating that LC50(96h) is > 999 mg/l and NOEC = 999 mg/l of TFA. All validity criteria were fulfilled, i.e. mortality in fish control < 10%, constant conditions were maintained throughout the test, dissolved oxygen concentration was >= 60% of the air saturation value throughout the test, and tested concentration has been maintained for at least 80% of the nominal concentration throughout the test.

Considering that the reaction mass of chlorodifluoroacetic acid (CDFA) and trifluoroacetic acid (TFA) is mainly composed of CDFA which is the more toxic of the two constituents, the reaction mass is considered as harmful to fish.