Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-05-28 to 1990-06-15
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline-conform study under GLP. Deviations made are explained and considered being acceptable concerning the overall quality of the study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
see below
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
yes
Remarks:
see below
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Cyprinus carpio

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
not applicable

Test conditions

Hardness:
11.8 °DH
Test temperature:
21 to 21.5 °C.
pH:
8.2 +/- 0.2
Dissolved oxygen:
Oxygen concentration in the test media was found to be > 5 mg/l for all measurements performed during the final study.
Salinity:
Not being reported as such, however the detailed mineral composition of the used so-called "Dutch standard water" (DSW) is given in the original study report.
Nominal and measured concentrations:
Measured concentration varied between 93 and 106 % of the nominal concentrations of the test item.
Reference substance (positive control):
yes
Remarks:
pentachlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 180 - <= 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: Concentrations: 0.18, 0.32, 0.56, 1.0 and 1.8 mgll i n DSW. At concentrations at 0.56 mg/l and higher All fish died within 96 hours
- LC50: ca. 0.5 mg/l
- Other:
Reported statistics and error estimates:
no statistics applied

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96h-LC50 for carp exposed to the substance was between 180 and 320 mg/l with 0% mortality at 100 mg/l and 100% mortality at 320 mg/l.