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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

96h LC50 (Danio rerio) = 5.3 mg/L (nominal concentration, ISO 7346-1)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
5.3 mg/L

Additional information

Since no short-term toxicity test with fish is available for C8-14AS NH4 & TEA (CAS 96690-74-3), in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to other structurally related category member was conducted. Read across data from another C8-14 alkyl sulfate was used: C8-14AS NH4 (CAS 90583-10-1) and C8-14AS TEA (CAS 85665-45-8).

A GLP short-term fish toxicity test with C8-14AS NH4 (CAS 90583-10-1) was performed according to ISO 7346-1 guideline using zebra fish (Guhl 1992). Mortality of fish was determined under static conditions after 24, 48, 72 and 96 hours of exposure to the concentrations up to 32 mg product/L (nominal concentration) of 32.9% pure technical product in water. The nominal concentrations were in agreement with the measured ones. The results showed that the 96-hour LC50 value was 5.3 mg a.i./L (nominal concentration). The composition of the read-across substance C8-14AS NH4 (CAS 90583-10-1) used in the short-term fish experiment was: C8: 12%, C10: 12%, C12: 53%, C14: 23%.

Moreover, a supporting short-term fish toxicity test is available for C8-14AS TEA (CAS 85665-45-8; Richterich 2003). The test was performed with golden orfe (Leuciscus idus) according to DIN 38412 - L15 guideline. Mortality of fish caused by the test substance was determined after 24 and 48 hours of a static exposure to the concentration up to 64 mg/L of 46.8% pure technical product. The results showed that the 48-hour LC50 value was 8.9 mg a.i./L. The composition of the read-across substance C8-14AS TEA (CAS 85665-45-8) used in the short-term fish experiment was: C8: 8.5%, C10: 8.5%, C12: 68%, C14: 25%, C16: 6%.

Due to structural and property similarities with this category member the above results can also be applied for C8-14AS NH4 & TEA (CAS 96690-74-3). The composition of this substance based on the mean values of provided ranges is: 14.1% of C8; 14.1% of C10; 46.9% of C12, 24.9% of C14. The reliable 96-hour LC50 value for fish is therefore 5.3 mg/L.