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EC number: 200-087-7 | CAS number: 51-28-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data have been obtained from secondary source.
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 1 995
- Reference Type:
- review article or handbook
- Title:
- Effect of some phenolic compounds on chromosomes of bone marrow cells in mice
- Author:
- Mitra AB, Manna GK.
- Year:
- 1 971
- Bibliographic source:
- Indian J Med Res 59:1442-1447.
Materials and methods
- Principles of method if other than guideline:
- The method is based on a chromosome aberration assay in vivo. An intraperitoneal injection of three saturated solution concentrations was made on mice. After 24 hours bone marrow cells were analyzed. No further details were specified.
- GLP compliance:
- no
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- 2,4-dinitrophenol
- EC Number:
- 200-087-7
- EC Name:
- 2,4-dinitrophenol
- Cas Number:
- 51-28-5
- Molecular formula:
- C6H4N2O5
- IUPAC Name:
- 2,4-dinitrophenol
- Test material form:
- other: saturated solution
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
Administration / exposure
- Route of administration:
- intraperitoneal
- Post exposure period:
- Mice were sacrified 24 hours posttreatment for analysis of bone marrow cells.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0.25 ml
Basis:
other: saturated solution
- Remarks:
- Doses / Concentrations:
0.5 ml
Basis:
other: saturated solution
- Remarks:
- Doses / Concentrations:
1 ml
Basis:
other: saturated solution
Examinations
- Tissues and cell types examined:
- Bone marrow cells was observed for chromosomal aberration analysis.
Results and discussion
Test results
- Sex:
- not specified
- Genotoxicity:
- positive
- Toxicity:
- not specified
Any other information on results incl. tables
The authors concluded that 2,4-DNP was clastogenic under the assay conditions and attributed the effect to the compound’s electrophilic properties.
However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
2,4-DNP was clastogenic under the assay conditions due to compound’s electrophilic properties. However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol. - Executive summary:
Data have been obtained from secondary source that mentions Mitra AB, Manna GK. 1971. Effect of some phenolic compounds on chromosomes of bone marrow cells in mice. Indian J Med Res 59:1442-1447.
The method is based on a chromosome aberration assay in vivo. Mice were injected intraperitoneally with 0.25, 0.50, and 1 mL of a saturated solution of 2,4-DNP, then sacrificed 24 hours posttreatment for analysis of bone marrow cells for chromosomal aberrations . No further details were specified.
The authors concluded that 2,4-DNP was clastogenic under the assay conditions and attributed the effect to the compound’s electrophilic properties.
However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.
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