Sodium 3-(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-04-11 to 1988-04-15

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

non-GLP

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Paris 1984

Deviations:

yes

Remarks:

Holding: in dechlorinated tap water; Test: in artifical water; Temperature: higher temperature variation

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

EEC directive, Annex V, C.1

Deviations:

yes

Remarks:

Holding: in dechlorinated tap water; Test: in artifical water; Temperature: higher temperature variation

Principles of method if other than guideline:

NA

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Analytical monitoring:

no

Details on sampling:

NA

Vehicle:

no

Details on test solutions:

The test substance was dissolved in 200 mL water, which were slowly added to the fish bath.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish (Brachidanio rerio)
- Source: West Aquarium, Bad Lauterberg, BRD
- Age at study initiation (mean and range, SD): 18 weeks
- Length at study initiation (length definition, mean, range and SD): 25,3 mm
- Weight at study initiation (mean and range, SD): 0,15 g
- Feeding: none

ACCLIMATION
- Acclimation period: 12 days in dechlorinated tap water

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

NA

Hardness:

hardness of 100 mg/L (as CaCO3)

Test temperature:

23.1 - 24.5°C

pH:

pH= 7.7-8.2

Dissolved oxygen:

O2= 7.7 to 8.3 mg/L

Salinity:

NA

Nominal and measured concentrations:

100, 178, 316, 562 and 1000 ppm (=mg/L);
- Conc. start: 102, 177, 317, 559, 1000 ppm
- Conc. end: 103, 179, 324,566, 984 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 liter glass aquaria
- Volume: 2.9 litre

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

420 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

316 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

562 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No abnormal responses of the fish were observed.

Results with reference substance (positive control):

NA

Reported statistics and error estimates:

NA

Sublethal observations / clinical signs:

Table of the test concentration of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate:

 

 

Nominal conc. ppm	Conc. Start		Conc. End
	ppm	% of nominal	ppm	% of nominal
100	102	102	103	103
178	177	99	179	101
316	317	100	324	103
562	559	99	566	101
1000	1000	100	984	98

 

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (96 h) of sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was 420 mg/L.

Executive summary:

Sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was tested in an acute toxicity test with Zebrafish (Brachidanio rerio) under semi-static conditions according to OECD guideline 203 and EEC directive C.1.

Groups of ten fish were exposed to test item at nominal concentration of 100, 178, 316, 562 and 1000 mg/L. A control was carried out with water (blank control).

The LC50 after 96 h was 420 mg/L . The LC0 and LC100 after 96 h were 316 mg/L and 562 mg/L.

Description of key information

LC50(96h) = 420 mg/L for mortality of Danio rerio (OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

420 mg/L

Additional information

Sodium 3(2H-benzotriazol-2-yl)-5-sec-butyl-4-hydroxybenzenesulfonate was tested in an acute toxicity test with Zebrafish (Brachidanio rerio) under semi-static conditions according to OECD guideline 203 and EEC directive C.1.Groups of ten fish were exposed to test item at nominal concentration of 100, 178, 316, 562 and 1000 mg/L. A control was carried out with water (blank control). The LC50 after 96 h was 420 mg/L. The LC0 and LC100 after 96 h were 316 mg/L and 562 mg/L