Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-815-4 | CAS number: 126-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
For the evaluation of the biodegradability of thioglycolic acid and its salts, ready biodegradation and inherently biodegradation test results have been considered but also results obtained with dithiodiglycolate, the main oxidation product of the thioglycolate anion.
Thioglycolic acid (TGA):
Ready biodegradation tests
Van Ginkel and Stroo (1992; reliability 2): A method to prolong the closed bottle test up to 200 days has been implemented to prevent discrepancies and unexplainable results obtained in 28-day test. This test, when recognized by the Authorities, has the potential to be used as an inherent biodegradability test. Among screening tests, the closed bottle test has been retained by the authors as it is simple and it uses low initial concentration of test compound (interesting for toxic compounds). Activated sludge was used as an inoculum as described by Bloket al,1985 (reliability 2). The preconditioned and aerated sludge was diluted to a concentration of 2 mg/dry wt/L. Ammonium chloride was omitted from the medium to prevent nitrification. Thioglycolic acid was retained as candidate for the test based on discrepancies among results obtained by participants of an EEC round robin test. In this tailor-made test validity criteria are respected demonstrating that only very minor quantities of molecular oxygen are introduced into the bottle. Moreover, molecular oxygen quantity is sufficient enough in the frame of the prolonged test. Endogenous respiration at day 28 is 0.4 mg/L and 2.3 mg/L after approximately 200 days. Oxygen concentration does not fall below 0.5 mg/L during the test period.
In the frame of the test, thioglycolic acid is demonstrated as ready biodegradable with 67% biodegradation at day 28.
Bloket al.(1985; reliability 2): The authors point out discrepancies in the results obtained using different screening test procedures. Although these tests are performed according to standard protocols, the results obtained with each individual method for the same compound may, in fact, indicate a pass or fail on different occasions. For instance, in OECD round-robin test carried out from 1979 to 1980, the following results have been obtained for thioglycolic acid:
Ready biodegradation of thioglycolic acid in a round-robin test:
Tests ranked in order of stringency | Biodegradation percentages |
OECD 301 B | 60 |
OECD 301 C | 40 |
OECD 301 E | 13 |
OECD 301 D | 0 |
In the frame of the round-robin test, thioglycolic acid is demonstrated to be ready biodegradable with 60% biodegradation at day 28 using OECD TG 301B test. OECD TG 301C, E and D tests tend to demonstrate that thioglycolic acid is not ready biodegradable. It is expected to have two main causes of the variability of results obtained with the presently-accepted test protocols: those resulting from the analytical procedures chosen and those resulting from differences in biological criteria, those latest can be harmonized to some extent.
That is probably one of the reasons why one notes differences in results between Van Ginkel and Stroo, 1992 ( reliability 2) here above cited and closed bottle test carried out in the round-robin test.
Painter and King (1985; reliability 2): A ring test programme has been initiated in 1983-1984 in order to assess the biodegradability of chemicals in water by manometric respirometry (OECD TG 301C). This has been carried out by 26 participants accustomed to the technique of respirometry. As for Bloket al,1985, this ring test intends to address the question of the discrepancy between the results obtained for one substance depending on the laboratory technique used. For thioglycolic acid, although the median delay time (td) was 10 days, only 5 out of 20 laboratories reached 60% ThOD at (td + 10) days, rising to 8/20 at 28d. Even if the ‘pass’ level was reduced to 40%, only one more laboratory would qualify (9/20). Since the mean proportion of ThOD at 28d was 43%, TGA should be considered as intermediate in biodegradability.
Boutonnet, 1994 (reliability 2): The author carried out a OECD 301A test. It has to be noted that cells concentration used in the test was 4 x 105bact/mL, i.e. 4 x 103bact/mL in the final inoculated medium. This value is below the recommended inoculum (104to 105cell/mL). It does not invalidate the assay but it is not in favor of substance degradation. The thioglycolic acid concentration tested was 40 mg DOC/L. The test concludes that the thioglycolic acid is not toxic to bacteria, no abiotic degradation is observed and thioglycolic acid cannot be considered as ready biodegradable based on the final result obtained after the 28-day period, i.e. 21%. Nevertheless 2 elements have to be taken into consideration: (i) stringency of the method which does not necessarily preclude ready biodegradation potential, (ii) lower inoculum concentration which potentially impacts the degradation level.
Bloket al.(1985; reliability 2): In the frame of the round-robin test carried out between 1979 and 1980 and already described here above, inherent biodegradation tests were also performed. The following results have been obtained for thioglycolic acid:
Inherent biodegradation of thioglycolic acid in a round-robin test
Tests ranked in order of stringency | Biodegradation [in %] |
OECD TG 302B | 67 |
OECD TG 302C | 60 |
In the frame of the round-robin test, thioglycolic acid is demonstrated to be inherently biodegradable with 60% biodegradation at day 28 using OECD TG 302C test.
Chemicals Inspection Testing Institute (1992; reliability 4): A biodegradation study on thioglycolic acid,based on BOD measurements, using an activated sludge seed, and an initial chemical concentration of 30 mg/L indicated 100% biodegradation after 4 weeks. This result would indicate that thioglycolic acid is ready biodegradable, but the report is not available for assessment.
Results obtained in the frame of inherent biodegradation tests confirm without any doubt the fact that the thioglycolate anion is inherently biodegradable.
The results mentioned demonstrate results discrepancies depending test uses and specific experimental conditions.
Nevertheless physico-chemical processes have to be considered for the general picture. Half-life of thioglycolic acid is shown to be short due to oxidation process. On this basis, it makes sense to focus on the main oxidation product, the.
Monoethanolamine thioglycolate (MeaTG)
Biodegradation of MeaTG has been investigated by using OECD TG 301B and GLP requirements. MeaTG was exposed to activated sewage sludge microorganisms at a concentration of 5 mg C/L with culture medium in sealed culture vessels in the dark at 21 °C for 28 days. The degradation of MeaTG was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
The test material attained 117% degradation after 28 days. Under the strict terms and conditions of OECD TG 301B the test material can be considered to be readily biodegradable as the test material satisfied the 10-day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation rate exceeding 10%. All the validation criteria for this test were satisfied.
Diammonium dithiodiglycolate (DADTDG, ammonium salt of dithiodiglycolate):
A study was performed to assess the ready biodegradability of diammonium dithiodiglycolate (CAS No. 68223-93-8) in an aerobic aqueous medium (Clarke, 2005; reliability 2) following OECD TG 301B, "Ready Biodegradability; CO2-Evolution Test.
An initial experiment was conducted at a concentration of 10 mg C/L, however, inhibition of CO2evolution in the test vessels was observed after 8 days. Therefore, following the recommendations of the test guideline, DADTDG was exposed to activated sewage sludge micro-organisms, at a concentration of 5 mg C/L with culture medium in sealed culture vessels in the dark at 21 °C for 28 days. The degradation of DADTDG was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the standard material, sodium benzoate, together with a toxicity control were used for validation purposes.
The test material attained 80% degradation after 28 days. Under the strict terms and conditions of OECD TG 301B the test material cannot be considered to be readily biodegradable as the test material failed to satisfy the 10-day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation rate exceeding 10%.
However, the test material has exhibited the potential for rapid degradation. All the validation criteria for this test were satisfied.
Considering the overall information available for the thioglycolate anion and its main oxidation product, the dithiodiglycolate, it can be considered that thioglycolic acid and its salts are readyly biodegradable and do not raise concern in terms of persistency.
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live1