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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Qualifier:
equivalent or similar to
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Remarks:
HPLC-UV

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Strain No. 61.81 SAG.
The algae were supplied by the "Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen", 37073 Göttingen, Germany.

Study design

Test type:
static
Water media type:
other: reconstituted water (OECD medium)
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
Microscopic examination of the shape of the algal cells after 72 hours.
Determination of cell density by spectrophotometrical measurement after 24h, 48h, 72h.

Test conditions

Hardness:
0,24 mmol/L (=24 mg/L) as CaCO3
Test temperature:
21.5 to 23.5°C (water temperature)
pH:
6.0 to 6.1
Dissolved oxygen:
Not specified
Nominal and measured concentrations:
Nominal: 100, 32, 10, 3.2, 1.0, 0.32 and 0.10 mg test item/L
Measured: 94.9, 27.4, 7.07, 2.37, 0.774, 0.267 and 0.088 mg test item/L
Details on test conditions:
Continuous illumination (mean light intensity: 5140 lux)
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 9.15 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 0.774 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
ca. 2.37 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 2.34 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 0.774 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
ca. 2.37 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
other: yield

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
All reported results refer to geometric mean concentrations, since the test item concentrations were not within +/- 20% of the nominal concentrations during the test.
Based on the experimental results, the 72-h EyC50 (yield) was calculated to be 2.34 mg test item/L and the 72-h ErC50 (growth rate) was calculated to be 9.15 mg test item/L.
The 72-h NOEyC (yield) was determined to be 0.774 mg test item/L and the associated 72-h LOEyC (yield) was 2.37 mg test item/L.
The 72-h NOErC (growth rate) was determined to be 0.774 mg test item/L and the associated 72-h LOErC (growth rate) was 2.37 mg test item/L.