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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
<= 28 d
Initial test substance concentration
Initial conc.:
ca. 102.3 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The test item Pyrazol was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was fol- lowed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Test conditions: 22°C ± 1°C, darkness, climatised chamber, pH of 7.4 to 7.6 (measured at the start of the test) and 7.4 to 7.5 (measured at the end of the test)
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Key result
Parameter:
% degradation (O2 consumption)
Value:
ca. 2.5
Sampling time:
28 d
Remarks on result:
other: based on ThOD(NH4)
Key result
Parameter:
% degradation (O2 consumption)
Value:
ca. 2
Sampling time:
28 d
Remarks on result:
other: based on ThOD(NO3)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The test item did never reach 10% degradation during incubation time. The mean biodegradation at test end after 28 days was 2.5% (ThODNH4) and 2% (ThODNO3).
Therefore, Pyrazol is considered to be not readily bio- degradable based on ThODNH4 / ThODNO3.