Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 274-713-2 | CAS number: 70643-20-8
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Based on the nominal concentrations of the test item 2,4-diaminophenoxyethanol sulphate dihydrate the 48 hour EC50 for Daphnia magna was estimated to be 7.40 mg/L (95% confidence limits : 6.64 - 8.24 mg/L). The NOEC after 48 hours was determined to be 2.50 mg/L as the highest tested concentration with no biologically significant effect. The LOEC after 48 hours was determined to be 5.00 mg/L as the lowest tested concentration with a biologically significant effect. Immobilisation rates of >10% are seen to be significant according to the guideline.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 7.4 mg/L
Additional information
In the acute immobilization test with Daphnia magna the effects of the test item 2,4-diaminophenoxyethanol sulphate dihydrate were determined according to OECD guideline 202. The study was conducted under semi-static conditions with 6 concentrations ranging from 1.25 to 40.0 mg/L set up in a geometric series with a dilution factor of 2 over a period of 48 hr. Twenty daphnids were exposed to each test concentration and the control. Concentrations of the test item were determined in the fresh media (0 and 24 hr) and old media (24 and 48 hr) of all tested concentration levels and the control by HPLC. The recovery rates of 2,4-diaminophenoxyethanol sulphate dihydrate in the fresh media (0 and 24 hr) were in the range of 100 to 107% of the nominal values at all tested concentration levels. The recovery rates in the old media (24 and 48 hr) were in the range of 97 to 106% of the nominal values. The water quality parameters (pH value and dissolved oxygen concentration), measured at 0, 24 and 48 hr, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled. Based on the nominal concentrations of the test item 2,4-diaminophenoxyethanol sulphate dihydrate the 48 hour EC50 for Daphnia magna was estimated to be 7.40 mg/L (95% confidence limits : 6.64 - 8.24 mg/L). The NOEC after 48 hours was determined to be 2.50 mg/L as the highest tested concentration with no biologically significant effect. The LOEC after 48 hours was determined to be 5.00 mg/L as the lowest tested concentration with a biologically significant effect. Immobilisation rates of >10% are seen to be significant according to the guideline.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
