hexasodium 4-[3-(2-bromoprop-2-enamido)benzamido]-6-[(E)-2-[5-(2,3-dibromopropanamido)-2-sulfophenyl]diazen-1-yl]-5-hydroxynaphthalene-1,7-disulfonate 6-[(E)-2-[5-(2-bromoprop-2-enamido)-2-sulfophenyl]diazen-1-yl]-4-[3-(2-bromoprop-2-enamido)benzamido]-5-hydroxynaphthalene-1,7-disulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

The test was done at Ciba-Geigy internal labs according to a standard SOP comparable to OECD 203 as of today before any international guidelines have been established.

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Details on sampling:

No data

Vehicle:

no

Details on test solutions:

No data

Test organisms (species):

other: Fish (Trout)

Details on test organisms:

No data

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

14 °C

pH:

7.4 to 8.2

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

2, 20, 100, 200 and 1000 mg/l

Details on test conditions:

No data

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

450 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

None

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

None

Validity criteria fulfilled:

yes

Conclusions:

48 h LC50 for FAT 40065/A is found to be 450 mg/l in trout fish.

Executive summary:

48 h LC50 for FAT 40065/A is found to be 450 mg/l in trout fish.

LC 0 = 200 mg/l

LC100 = 1000 mg/l

Description of key information

The LC50 to trout was 450 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

450 mg/L

Additional information

8 Rainbow trouts per test concentration have been tested in a pre-guideline test comparable to OECD 203 for acute fish toxicity. test reports done with test item FAT 40065/A are available. The nominal concentrations of FAT 40046/A tested were 2, 20, 100, 200 and 1000 mg/L. Additionally, a blank control without test item was carried out. The duration of the test was 48 hours. Based on the mortality occurred the following LC-values have been defined. Additional toxicity data beyond mortality have not been reported.

LC0 after 48 h was 200 mg/L, the LC50 was 450 mg/L (graphically determined) and LC100 after 48 h was 1000 mg/L.