Registration Dossier
Registration Dossier
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EC number: 213-116-3 | CAS number: 925-21-3
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LC50 value of 13 mg/L with 95% confidence limits of 8.7 - 19 mg/L. The Lowest Observed Effect Concentration was considered to be 10 mg/L. The No Observed Effect Concentration was 3.2 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 13 mg/L
Additional information
Introduction
A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.
Methods
Following preliminary range-finding tests and an initial experiment, fish were exposed, in groups of seven, to an aqueous solution of the test item over a range of concentrations of0.32,1.0,3.2,10and 32 mg/L for a period of 96 hours at a temperature of13 °C to14 °C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Results
The 96-Hour LC50based on nominal test concentrations was 13 mg/L with 95% confidence limits of 8.7 ‑ 19 mg/L. The Lowest Observed Effect Concentration was considered to be 10 mg/L. The No Observed Effect Concentration was 3.2 mg/L.
Analysis of the test preparations at 0, 24, 72 and 96 hours showed measured test concentrations to range from 84% to 119% of nominal and so the results are based on nominal test concentrations only.
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