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Short-term toxicity to aquatic invertebrates

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There is no aquatic invertebrate toxicity test for "Reaction products of 3-methylbutanal and 3-methylbut-3-en-1-ol, distillation overheads, acid-treated, hydrogenated" available. Thus, the hazard assessment is based on both main components of the Reaction products. For 2H-Pyran, tetrahydro-4-methyl-2-(2-methylpropyl)- (CAS 13477-62-8; 30 -80% of the Reaction product) a study of a analog substance (CAS 16409-43-1) has been used. The toxicity of this substance towards Daphnia magna was investigated according to OECD Guideline 202 under GLP conditions (BASF 2008). The calculated 48h-EC50 was 33.2 mg/l (nominal).

For the second main component 2H-pyran-4 -ol (CAS 63500-71-0; 10 -60% of reaction product) a

guideline study on the acute toxicity to Daphnia magna was performed according to OECD guideline 202 and an EC50 of ca. 320 mg/l was detected. The animals were exposed to the substance for 48 hours in a static test system (Unilever Research UK, 1996).

Based on the assessment of both main components, the toxicity to aquatic invertebrates of "Reaction products of 3-methylbutanal and 3-methylbut-3-en-1-ol, distillation overheads, acid-treated, hydrogenated" is considered to be 33.2 mg/L as a worst-case assumption.