(chloromethyl)triethoxysilane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-04-27 to 1994-04-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 500 mg/l nominal treatment

- Sampling method: Samples of test media were taken at the start and end of the test

- Sample storage conditions before analysis: Refrigerated

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: A 500 mg/l nominal concentration of the test substance was prepared by stirring overnight.

- Controls: Dilution water

Test organisms (species):

Daphnia magna

Details on test organisms:

- Type and amount of food: Chlorella vulgaris at 500000 cells/ml

- Feeding frequency: Daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

204 mg/l as CaCO3

Test temperature:

20.3 - 21.7ºC

pH:

7.4 - 8.1

Dissolved oxygen:

99 - 100% ASV

Nominal and measured concentrations:

Nominal: 0(Control), 500 mg/l

Measured concentration at start of test: 405 mg/l

Measured concentration at end of test: 436 mg/l

Mean measured concentration: 420 mg/l

Mean measured concentration as a percentage of nominal: 84.1%

The test results are presented and interpreted with reference to nominal concentrations.

Details on test conditions:

TEST SYSTEM

- Test vessel: Conical flasks

- Type: closed

- Material, fill volume: Glass, 150 ml of test medium

- Aeration: none

- No. of organisms per vessel: 5

- No. of vessels per concentration (replicates): 4

- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Reconstituted freshwater prepared by mixing (1:1) treated mains water and proprietary mineral water (Ashbourne, Still Natural Water (Nestle, UK. Limited).

- Culture medium different from test medium: no

- Chlorine: <0.1 mg/l (Total)

- Alkalinity: 134 mg/l as CaCO3

- Conductivity: 404 μS/cm

OTHER TEST CONDITIONS

- Adjustment of pH: no

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization after 24 and 48 h

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but exposure is to hydrolysis product

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but exposure is to hydrolysis products

Basis for effect:

mobility

Details on results:

- Mortality of control: 0

Reported statistics and error estimates:

No toxic effects were observed in the test media, the test results were therefore not subject to statistical analysis.

Validity criteria fulfilled:

yes

Conclusions:

A 48-hour EC50 value of >500 mg/l and NOEC of ≥500 mg/l have been determined for the effects of the test substance on mobility of Daphnia magna based on nominal concentration of the substance. However it is likely that the test organisms were primarily exposed to the hydrolysis products of the substance.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the attached justification for grouping of substances provided in IUCLID Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but exposure is to hydrolysis product

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

but exposure is to hydrolysis products

Basis for effect:

mobility

Description of key information

A 48-hour EC₅₀of > 500 mg/l, OECD 202, RL1

Additional information

Measured data was not available for (chloromethyl)triethoxysilane (CAS No. 15267-95-5) therefore read across from the comparable substances, triethoxy(methyl)silane (CAS No. 2031-67-6) was used to fill the data gap for invertebrates.

A 48-hour EC₅₀value of >500 mg/l and NOEC of ≥500 mg/l have been determined for the effects of the test substance on mobility of Daphnia magna based on nominal concentration of the substance. The study was conducted according to a standard guideline (EU Method C.2 (Acute Toxicity for Daphnia) and GLP and was selected as key study (Hazleton, 1994). However, it is likely that the test organisms were primarily exposed to the hydrolysis products of the substance.