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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

Activated sludge toxicity test, OECD 209, EC50 (3h) >= 10000 mg/L 

Key value for chemical safety assessment

EC50 for microorganisms:
10 000 mg/L

Additional information

The acute toxicity to microorganisms of the substance 2,4,6 -triisopropyl-m-phenylene diisocyanate was investigated in accordance with OECD Guideline 209 without GLP compliance (Kanne, 1989). Activated sludge was used in the static freshwater experiment with an exposure time to the test substance of 3 hours. The culture medium was prepared according to ISO 8192 -1986, Chapter 4.2. The temperature range was 20.5 - 21.1 °C (Control: 20.5 - 20.7 °C) and the pH was adjusted in the range of 7.4 - 7.8 (Control: 7.9). The dissolved oxygen was 5.4 - 8.7 mg at the beginning and 4.6 - 8.7 mg at the end of the experiment. The test substance was tested in the concentrations of 100, 1000 and 10000 mg/L. 3,5 -Dichlorphenol was used as reference substance and showed 66.7 % inhibition at a quantity of 5.00 mg. Regarding the test substance an inhibition of 11.1 % was reported at a quantity of 2500 mg, which reveals a concentration of 10000 mg/L. Additionally, an EC50 (3h) value of >= 10000 mg/L is reported as result, but with the remark of water insolubility.