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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

LC50(96h): 5621 mg/L (Danio rerio, EU Method C.1) 
(Read-across approach: Pimephales promelas, LC50(96h): 164.5 mg/L)

Key value for chemical safety assessment

LC50 for freshwater fish:
5 621 mg/L

Additional information

The acute toxicity of the test substance was investigated according to EU Method C.1 (which is in most parts equivalent to OECD guideline 203) (Bruns, 2002). No GLP compliance was cited. Danio rerio (Zebra fish) was used as test organisms and where exposed for 96 hours to the test substance. A control was included, no reference substance is mentioned. It was a static freshwater test with a temperature range of 21.2 °C - 22.0 °C (control: 21.6 °C - 21.9 °C) and a pH range of 7.2 - 7.8 (control: 7.4 - 7.9) depending on the nominal substance concentrations (1000, 3160 and 10000 mg/L). Direct weighing was the method of administration with a 16 hours light / 8 h dark cycle during the test period. As results a LC0 of 3160 mg/L is reported, as well as a LC50 of 5621 mg/L and a LC100 of 10000 mg/L. All values were reported after the exposition period of 96 hours. The study is regarded as a valid guideline study.

Furthermore, an acute toxicity study to fish of the read-across substance is available. In this case, Pimephales promelas was chosen as freshwater representative and was tested under static conditions followed the Commitee on Methods for Toxicity Tests with Aquatic Organisms (1975) Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians. U.S. EPA. Ecological Research Series EPA-660/3-75-009 (Curtis et al., 1978). The basic scientific principles of fish tests which will be performed today are met. The test duration was 96 h at a temperature of 22 +/- 1 °C without aeration and feeding during the test period. A series of substances were tested, whereby the substances were administered either directly or in the form of stock solutions in deionized water or acetone. The solutions were briefly stirred with a glass rod, then a water sample was withdrawn from one tank at each concentration for quantitative toxicant analysis. The test concentrations were arranged in geometric series with at least a 60 % dilution factor. As result, a LC50 value of 164.5 mg/L with 95 % confidence limits of 108.8 - 240.4 mg/L is reported.

In both cases, no indication to classify any of the substances as toxic is available.